Sandor Kasas

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Rapid detection of bacterial resistance to antibiotics using AFM cantilevers as nanomechanical sensors

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Livan Bladimir Alonso Sarduy, Justin Terence Notz Pekkanen, Laura Rio Marques

The widespread misuse of drugs has increased the number of multiresistant bacteria1, and this means that tools that can rapidly detect and characterize bacterial response to antibiotics are much needed in the management of infections. Various techniques, s ...

Nature Publishing Group2013

Nanoscale motion detector

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Livan Bladimir Alonso Sarduy

Motion detector comprising a flexible support (1,5) adapted to hold at least one object (6-9), a sensor (4) for measuring the displacement of said support (1) and processing means for differentiating the fluctuations of said support (1) from those induced ...

2013

Time-Lapse AFM Imaging of DNA Conformational Changes Induced by Daunorubicin

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Livan Bladimir Alonso Sarduy

Cancer is a major health issue that absorbs the attention of a large part of the biomedical research. Intercalating agents bind to DNA molecules and can inhibit their synthesis and transcription; thus, they are increasingly used as drugs to fight cancer. I ...

American Chemical Society2013

Combination of fluorescence microscopy and nanomotion detection to characterize bacteria

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Justin Terence Notz Pekkanen

Antibiotic-resistant pathogens are a major health concern in everyday clinical practice. Because their detection by conventional microbial techniques requires minimally 24h, some of us have recently introduced a nanomechanical sensor, which can reveal moti ...

Wiley-Blackwell2013

Stiffness tomography exploration of living and fixed macrophages

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Charles Roduit, Bhaskar Saha, Andrea Volterra

Stiffness tomography is a new atomic force microscopy imaging technique that allows highlighting structures located underneath the surface of the sample. In this imaging mode, such structures are identified by investigating their mechanical properties. We ...

Wiley-Blackwell2012

Force volume and stiffness tomography investigation on the dynamics of stiff material under bacterial membranes

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Charles Roduit, Laura Rio Marques

The determination of the characteristics of micro-organisms in clinical specimens is essential for the rapid diagnosis and treatment of infections. A thorough investigation of the nanoscale properties of bacteria can prove to be a fundamental tool. Indeed, ...

Wiley-Blackwell2012

Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth

Giovanni Dietler, Sandor Kasas, Charles Roduit, Christian Fankhauser

Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used ato ...

Elsevier2012

Differential peptide binding to CD40 evokes counteractive responses

Giovanni Dietler, Sandor Kasas, Charles Roduit, Livan Bladimir Alonso Sarduy, Bhaskar Saha

The antigen-presenting cell-expressed CD40 is implied in the regulation of counteractive immune responses such as induction of pro-inflammatory and anti-inflammatory cytokines interleukin (IL)-12 and IL-10, respectively. The mechanism of this duality in CD ...

Elsevier2012

Differential peptide binding to CD40 evokes counteractive responses (vol 73, pg 465, 2012)

Giovanni Dietler, Sandor Kasas, Charles Roduit, Livan Bladimir Alonso Sarduy, Bhaskar Saha

Elsevier2012

Determination of the size of quantum dots by fluorescence spectroscopy

Sandor Kasas

There has been a lack of quick, simple and reliable methods for determination of nanoparticle size. An investigation of the size of hydrophobic (CdSe) and hydrophilic (CdSe/ZnS) quantum dots was performed by using the maximum position of the corresponding ...

2011

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Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth

Giovanni Dietler, Sandor Kasas, Charles Roduit, Christian Fankhauser

Elsevier2012