Publications de Stefania Puttini

Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy

Giovanni Dietler, Harald Brune, Andrzej Kulik, Nicolas Mermod, Guillaume Witz, Stefania Puttini

Aim: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients. Materials & methods: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patient ...

Future Medicine2014

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Andrzej Kulik, Nicolas Mermod, Stefania Puttini, Déborah Ley, Damien Saugy

Background: The in vivo transfer of naked plasmid DNA into organs such as muscles is commonly used to assess the expression of prophylactic or therapeutic genes in animal disease models. Results: In this study, we devised vectors allowing a tight regulatio ...

BioMed Central2013

Intracellular nanomanipulation by a photonic-force microscope with real-time acquisition of a 3D stiffness matrix

Michaël Unser, László Forró, Giovanni Dietler, Andrzej Kulik, Philippe Thévenaz, Elena Bertseva, Gérard Gremaud, Sylvia Jeney, Stefania Puttini, Amardeep Singh

A traditional photonic-force microscope (PFM) results in huge sets of data, which requires tedious numerical analysis. In this paper, we propose instead an analog signal processor to attain real-time capabilities while retaining the richness of the traditi ...

Institute of Physics2009

Gene-mediated Restoration of Normal Myofiber Elasticity in Dystrophic Muscles

Andrzej Kulik, Nicolas Mermod, Stefania Puttini, Damien Saugy

Dystrophin mediates a physical link between the cytoskeleton of muscle fibers and the extracellular matrix, and its absence leads to muscle degeneration and dystrophy. In this article, we show that the lack of dystrophin affects the elasticity of individua ...

2009