Personne

David Hacker

Publications associées (68)

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Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

Florian Maria Wurm, Mohamed Farhat, David Hacker, Lucia Baldi Unser, Matthieu Dreyer, Stéphanie Tissot, Martino Reclari, Agata Oberbek

Bioprocesses for recombinant protein production with mammalian cells are typically controlled for several physicochemical parameters including the pH and dissolved oxygen concentration (DO) of the culture medium. Here we studied whether these controls are ...

Elsevier2011

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Lentivirus-derived vectors (LVs) were studied for the generation of stable recombinant Chinese hamster ovary (CHO) cell lines. Stable pools and clones expressing the enhanced green fluorescent protein (eGFP) were selected via fluorescence-activated cell so ...

Wiley-Blackwell2010

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiaowei Zhang, Isabel Fernandez Garcia

The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold rel ...

Springer Netherlands2010

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Valproic acid (VPA) is a small molecule that inhibits histone deacetylase activity. Here we report that VPA increases recombinant mRNA and protein levels in transiently transfected CHO DG44 cells. In the presence of VPA, transient recombinant antibody yiel ...

Elsevier2010

Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Mathieu Benjamin Stettler, Stéphanie Tissot, Xiaowei Zhang, Gilles Broccard

Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce a backwards and forwards 'wave' , or using orbital shaking to generate a 'wave' that runs round in a cylindrical container, have proved to be successful for ...

Schweizerische Chemische Gesellschaft2010

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Efficient mixing in bioreactors is essential in order to avoid concentration gradients which can be harmful for mammalian cells. To study mixing and its scalability in orbitally shaken cylindrical bioreactors, we measured mixing times in containers with no ...

Elsevier2010

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Gene transfer methods for producing recombinant cell lines are often not very efficient. One reason is that the recombinant DNA is delivered into the cell cytoplasm and only a small fraction reaches the nucleus. This chapter describes a method for microinj ...

Humana Press2009

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Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV f ...

2009

Transient Gene Expression in Mammalian Cells: Promises and Challenges for Medical Biotechnology

Florian Maria Wurm, David Hacker, Lucia Baldi Unser

Recombinant protein-based biologics are increasingly relevant in the pharmaceutical industry. Currently, mammalian cell-based bioprocesses are a routine manufacturing procedure for complex proteins such as recombinant antibodies, and the number of new biol ...

Wiley2009

25 years of recombinant proteins from reactor-grown cells — Where do we go from here?

Florian Maria Wurm, David Hacker

The purpose of this review is to describe the current status and to highlight several emerging trends in the manufacture of recombinant therapeutic proteins in cultivated mammalian cells. focusing on Chinese hamster ovary cells as the major production host ...

Elsevier2009