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Watch-dictaphone for Automatic Medical Codification

Pierre-André Farine, Sara Grassi Pauletti, Frédéric David Meylan, Patrick Stadelmann

This paper describes the development of a voice data recording watch and the accompanying software and hardware needed to build an integrated solution for medical data capture and automatic codification. The developed system is used to capture on the stand-alone watch, either by voice recording or by form-filling, the relevant information on the healthcare service being provided. The captured information is stored locally and then downloaded to a PC, via a USB connection. In the PC, speech is translated first into text and then into medical codification, with minimum userintervention. The resulting information is stored into local or centralized patient databases, to be sent later to 3rd-party SW for billing, reporting and statistics. The user controls the watch either via a touch-screen based interface or via a voice-driven interface for hands-free operation. Although this work is multidisciplinary, in this paper the emphasis is put on the description of the DSP implementation.

EURASIP2008

Optimized methods for the enzymatic ligation of oligoribonucleotides and analogues

Frédéric David Meylan

The preparation of oligoribonucleotides is of great interest for structural and functional investigations in biomolecular chemistry and biology. Relevant RNAs are relatively long and contain often modifications. Two methods for their preparation are currently available: automated chemical synthesis and in vitro transcription. The chemical synthesis allows the preparation of relatively short (< 60mers) pure RNA sequences containing modified nucleotides. In contrast, in vitro transcription is appropriate for the synthesis of long RNA sequences but does not allow the incorporation of modifications. An elegant solution to this dilemma is offered by enzymatic ligations of RNA fragments with T4-DNA ligase, which is template-dependent and therefore highly selective. Here, we present a comprehensive study of parameters, which govern the effectiveness of this ligation. The influence of secondary structures present within templates were studied in detail. That allowed to design a new type of hybrid template, containing both DNA and 2'-OMeRNA nucleotides, which were particularly efficient (Chapter 3.2). Ligation reactions with structured substrates and different types of oligonucleotides were carried out, leading to a better comprehension of the enzyme mechanism. In this context, we found very strong indications for a different recognition of the donor and acceptor substrates by the enzyme (Chapter 3.2 and 3.3). Optimization of the enzymatic ligations with different substrates or templates has showed the importance of secondary structures for design of a successful reaction (Chapter 3.2 and 3.3). The lessons drawn from these preliminary experiments have been employed for the reliable preparative synthesis of natural oligoribonucleotides containing modifications (Chapter 3.4) and the preparation of long RNA sequences (Chapter 3.5).

EPFL2006

Preparation of modified RNA sequences for biological research

Damian Ackermann, Frédéric David Meylan, Evelyne Muller, Sébastien Porcher, Luc Reymond, Philipp Wenter

Our group is working on the development of reliable access to biol. relevant, long, and modified RNA sequences. Here, we briefly present the chem. synthesis of such sequences with 2'-O-triisopropylsilyloxymethyl (= 2'-O-tom) protected ribonucleoside phosphoramidites, their template-directed enzymic ligation, and some examples of modified nucleotides, designed to promote structural and biol. studies. [on SciFinder (R)]

2005