Publications de Pascal Jourdain

Image-Based Marker-Free Screening of GABA(A) Agonists, Antagonists, and Modulators

Gerardo Turcatti, Benjamin Rappaz, Fabien Kuttler, Damiano Banfi, Pierre Marquet, Pascal Jourdain

The ionotropic GABA(A) receptors represent the main target for different groups of widely used drugs having hypnotic and anxiolytic effects. So far, most approaches used to assess GABA activity involve invasive low -throughput electrophysiological techniqu ...

2019

Mapping of real-time morphological changes in the neuronal cytoskeleton with label-free wide-field second-harmonic imaging: a case study of nocodazole

Sylvie Roke, Carlos Alberto Macias Romero, Pascal Jourdain, Marie Eve Pascale Didier, Claire Jacqueline Léa Teulon

We demonstrate the use of wide-field high-throughput second-harmonic (SH) microscopy for investigating cytoskeletal morphological changes on the single-cell level. The method allows for real-time, in vitro, label-free measurements of cytoskeletal changes t ...

SPIE-Intl Soc Optical Eng2019

Membrane water for probing neuronal membrane potentials and ionic fluxes at the single cell level

Pierre Magistretti, Sylvie Roke, Orly Bagunu Tarun, Pascal Jourdain, Marie Eve Pascale Didier

Neurons communicate through electrochemical signaling within a complex network. These signals are composed of changes in membrane potentials and are traditionally measured with the aid of (toxic) fluorescent labels or invasive electrical probes. Here, we d ...

Nature Publishing Group2018

Dual action of L-Lactate on the activity of NR2B-containing NMDA receptors: from potentiation to neuroprotection

Pierre Magistretti, Igor Allaman, Pierre Marquet, Pascal Jourdain, Kaspar Rothenfusser

L-Lactate is a positive modulator of NMDAR-mediated signaling resulting in plasticity gene induction and memory consolidation. However, L-Lactate is also able to protect neurons against excito-toxic NMDAR activity, an indication of a mitigating action of L ...

Nature Research2018

Synergistic interaction of L-Lactate and Glycine to potentiate NMDA receptor activity: a new form of meta-potentiation?

Pierre Magistretti, Igor Allaman, Pierre Marquet, Pascal Jourdain, Kaspar Rothenfusser

Wiley2017

Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

Pierre Magistretti, Christian Depeursinge, Benjamin Rappaz, Pierre Marquet, Pascal Jourdain, Kaspar Rothenfusser

Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation ...

Spie-Int Soc Optical Engineering2016

L-Lactate protects neurons against excitotoxicity: implication of an ATP-mediated signaling cascade

Pierre Magistretti, Igor Allaman, Pierre Marquet, Pascal Jourdain, Kaspar Rothenfusser

Converging experimental data indicate a neuroprotective action of L-Lactate. Using Digital Holographic Microscopy, we observe that transient application of glutamate (100 μM; 2 min) elicits a NMDA-dependent death in 65% of mouse cortical neurons in culture ...

Nature Research2016

Astrocyte swelling in response to neural activation: role of cotransporters

Pierre Magistretti, Pierre Marquet, Pascal Jourdain, Daniel Boss, Kaspar Rothenfusser

Wiley-Blackwell2015

High throughput second harmonic imaging for label-free biological applications

Pierre Magistretti, Sylvie Roke, Aleksandra Radenovic, Carlos Alberto Macias Romero, Pascal Jourdain, Orly Bagunu Tarun, Pierre Marquet, Vitalijs Zubkovs, Marie Eve Pascale Didier

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging th ...

Optical Society of America2014

The human CFTR protein expressed in CHO cells activates aquaporin-3 in a cAMP-dependent pathway: study by digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Pascal Jourdain, Sylvain Eric Lengacher

The transmembrane water movements during cellular processes and their relationship to ionic channel activity remain largely unknown. As an example, in epithelial cells it was proposed that the movement of water could be directly linked to cystic fibrosis t ...

Company of Biologists2014