Publications de Nicolas Dénervaud

Two distinct promoter architectures centered on dynamic nucleosomes control ribosomal protein gene transcription

Felix Naef, Sebastian Maerkl, Jacques Rougemont, Philippe Jacquet, Nicolas Dénervaud, Marcel Geertz

In yeast, ribosome production is controlled transcriptionally by tight coregulation of the 138 ribosomal protein genes (RPGs). RPG promoters display limited sequence homology, and the molecular basis for their coregulation remains largely unknown. Here we ...

Cold Spring Harbor Laboratory Press2014

Mapping the fine structure of a eukaryotic promoter input-output function

Sebastian Maerkl, Nicolas Dénervaud, Arun Stephen Rajkumar

The precise tuning of gene expression levels is essential for the optimal performance of transcriptional regulatory networks. We created 209 variants of the Saccharomyces cerevisiae PHO5 promoter to quantify how different binding sites for the transcriptio ...

Nature Publishing Group2013

A chemostat array enables the spatio-temporal analysis of the yeast proteome

Michaël Unser, Felix Naef, Sebastian Maerkl, Nicolas Dénervaud, Ricard Delgado Gonzalo, Johannes Becker, Arun Stephen Rajkumar

Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min t ...

National Academy of Sciences2013

A microfluidic live-cell imaging platform to study large collections of microbial genotypes

Nicolas Dénervaud

EPFL2013

KRAB-Zinc Finger Proteins and KAP1 Can Mediate Long-Range Transcriptional Repression through Heterochromatin Spreading

Didier Trono, Giovanna Ambrosini, Nadine Fournier, Philipp Bucher, Anna Claire Groner, Nicolas Dénervaud, Sylvain Meylan

Krüppel-associated box domain-zinc finger proteins (KRAB-ZFPs) are tetrapod-specific transcriptional repressors encoded in the hundreds by the human genome. In order to explore their as yet ill-defined impact on gene expression, we developed an ectopic rep ...

Public Library of Science2010

Multi-Target Tracking Of Packed Yeast Cells

Michaël Unser, Sebastian Maerkl, Ricard Delgado Gonzalo, Nicolas Dénervaud

The tracking of cell populations in time-lapse microscopy images enables high-throughput spatiotemporal measurements of cell dynamics. In this paper, we present a new algorithm to simultaneously track many cells in crowded areas. The algorithm runs in real ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2010