Publications de Christian Heinis

Structure‐affinity relationship analysis and affinity maturation of a calprotectin‐binding peptide

Christian Heinis, Cristina Diaz Perlas, Lluc Farrera Soler

Peptide 3 is an 18‐amino acid linear peptide binding with sub‐micromolar affinity to the inflammation marker calprotectin. Its application in point‐of‐care diagnostic assays has shown promising results, yet improving its affinity for calprotectin could fac ...

Wiley2025

Bulk measurement of membrane permeability for random cyclic peptides in living cells to guide drug development

Christian Heinis, Anne Sofie Luise Zarda, Alexander Lund Nielsen

Cyclic peptides are attractive for drug discovery due to their excellent binding properties and the potential to cross cell membranes. However, by far not all cyclic peptides are cell permeable, and measuring or predicting their membrane permeability is no ...

Wiley2025

Phage Display Selection against a Mixture of Protein Targets

Christian Heinis, Xudong Kong

Affinity selections by phage display or other display techniques are typically performed against single targets immobilized as a purified protein. In order to develop cross-specific binders that engage with multiple proteins, such as members of a related f ...

American Chemical Society (ACS)2025

Biodiversity2Drugs—Renaissance of exploring nature‐derived peptides for GPCR ligand discovery

Christian Heinis

Wiley2025

Discovery of De Novo Macrocycle Inhibitors of Histone Deacetylase 11

Christian Heinis, Anne Sofie Luise Zarda, Alexander Lund Nielsen, Edward Jeffrey Will

Histone deacetylase (HDAC) enzymes are epigenetic regulators that affect diverse protein function by removing acyl groups from lysine side chains throughout the proteome. The most recently discovered human isozyme, HDAC11, differs from other HDACs in subst ...

2025

Screening macrocyclic peptide libraries by yeast display allows control of selection process and affinity ranking

Christian Heinis, Edward Jeffrey Will, Lluc Farrera Soler

Springer Science and Business Media LLC2025

Solid-phase peptide synthesis in 384-well plates

Christian Heinis, Anne Sofie Luise Zarda, Alexander Lund Nielsen, Sevan Mleh Habeshian, Gontran Sangouard, Mischa Schüttel, Edward Jeffrey Will

Newer solid-phase peptide synthesis and release strategies enable the production of short peptides with high purity, allowing direct screening for desired bioactivity without prior chromatographic purification. However, the maximum number of peptides that ...

2024

Cyclic Peptides for Drug Development

Christian Heinis, Xinjian Ji, Alexander Lund Nielsen

Cyclic peptides are fascinating molecules abundantly found in nature and exploited as molecular format for drug development as well as other applications, ranging from research tools to food additives. Advances in peptide technologies made over many years ...

Wiley-VCH Verlag GmbH2024

Large Libraries of Structurally Diverse Macrocycles Suitable for Membrane Permeation

Christian Heinis, Xinjian Ji, Anne Sofie Luise Zarda, Alessandro Angelini, Alexander Lund Nielsen, Manuel Leonardo Merz, Ganesh Kumar Mothukuri, Mischa Schüttel, Zsolt Bognár, Edward Jeffrey Will

Macrocycles offer an attractive format for drug development due to their good binding properties and potential to cross cell membranes. To efficiently identify macrocyclic ligands for new targets, methods for the synthesis and screening of large combinator ...

Wiley-VCH Verlag GmbH2024

Peptide-Hypervalent Iodine Reagent Chimeras: Enabling Peptide Functionalization and Macrocyclization

Jérôme Waser, Christian Heinis, Xinjian Ji, Xingyu Liu

Swiss Chemical Soc2023