Publications de Gaurav Backliwal

Development of high-titer transient gene expression processes for manufacturing recombinant proteins

The market for recombinant therapeutic proteins (including antibodies) is estimated to be greater than $50-billion and has a compounded annual growth rate of around 20%. More than 50% of all approved processes for manufacturing recombinant proteins use mam ...

EPFL2008

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet

Recombinant proteins are of great commercial and scientific interest. However, most current production methods using mammalian cells involve the time- and labor-intensive step of creating stable cell lines. Although production methods based on transient ge ...

2008

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet, Sarah Wulhfard

Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stable cell lines. Here, we report an improved transient transfec ...

2008

High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI

Florian Maria Wurm, Gaurav Backliwal

2007

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery

Florian Maria Wurm, David Hacker, Martin Jordan, Martin Bertschinger, Gaurav Backliwal

Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various PEIs, allowed to mature for 10 min, and then exposed to different competitors (RNA, DNA, BSA or hepa ...

2006

Polyethylenimine-based quality control assay for plasmid DNA

Florian Maria Wurm, David Hacker, Martin Jordan, Martin Bertschinger, Gaurav Backliwal

2006