Publications de Nina Buffi | EPFL Graph Search

An automated microreactor for semi-continuous biosensor measurements

Philippe Renaud, Harald Van Lintel, Nina Buffi, Jan Roelof van der Meer

Living bacteria or yeast cells are frequently used as bioreporters for the detection of specific chemical analytes or conditions of sample toxicity. In particular, bacteria or yeast equipped with synthetic gene circuitry that allows the production of a rel ...

Royal Society of Chemistry2016

Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

Philippe Renaud, Harald Van Lintel, Nina Buffi

We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentrat ...

Amer Inst Physics2014

Bioreporters and biosensors for arsenic detection. Biotechnological solutions for a world-wide pollution problem

Philippe Renaud, Nina Buffi

A wide variety of whole cell bioreporter and biosensor assays for arsenic detection has been developed over the past decade. The assays permit flexible detection instrumentation while maintaining excellent method of detection limits in the environmentally ...

Elsevier2013

Design and construction of a portable whole-cell living bacterial biosensor for the detection of arsenic in water

Nina Buffi

Contamination of drinking water with arsenic is a recurring problem in both industrialized and developing countries. Supplies for large populations can have concentrations much higher than the permissible levels, set by the World Health Organization (WHO) ...

EPFL2013

Cell viability assessment by flow cytometry using yeast as cell model

Philippe Renaud, Nina Buffi, Niccolo Piacentini, Raphaël Tornay

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicated experimental setups and labeled samples. The use of ...

2011

Miniaturized bacterial biosensor system for arsenic detection holds great promise for making integrated measurement device

Philippe Renaud, Harald Van Lintel, Nina Buffi

Combining bacterial bioreporters with microfluidics systems holds great promise for in-field detection of chemical or toxicity targets. Recently we showed how Escherichia coli cells engineered to produce a variant of green fluorescent protein after contact ...

2011

Development of a microfluidics biosensor for agarose-bead immobilized Escherichia coli bioreporter cells for arsenite detection in aqueous samples

Philippe Renaud, Harald Van Lintel, Nina Buffi

Contamination with arsenic is a recurring problem in both industrialized and developing countries. Drinking water supplies for large populations can have concentrations much higher than the permissible levels (for most European countries and the United Sta ...

2011