Publications de Aline Roch | EPFL Graph Search

In Vivo Pre-Instructed HSCs Robustly Execute Asymmetric Cell Divisions In Vitro

Matthias Lütolf, Aline Roch, Mukul Girotra, Vincent Trachsel

Hematopoietic stem cells (HSCs) are responsible for life-long production of all mature blood cells. Under homeostasis, HSCs in their native bone marrow niches are believed to undergo asymmetric cell divisions (ACDs), with one daughter cell maintaining HSC ...

2020

Smart Hydrogels for the Augmentation of Bone Regeneration by Endogenous Mesenchymal Progenitor Cell Recruitment

Matthias Lütolf, Olaia Maria Naveiras Torres-Quiroga, Aline Roch, Philipp Sebastian Lienemann, Stéphanie Elisabeth Metzger, Queralt Vallmajó Martín, Martin Ehrbar, Vincent Milleret, Manuel Koch, Panagiota Papageorgiou

The treatment of bone defects with recombinant bone morphogenetic protein-2 (BMP-2) requires high doses precluding broad clinical application. Here, a bioengineering approach is presented that strongly improves low-dose BMP-2-based bone regeneration by mob ...

WILEY2020

Machine Learning of Hematopoietic Stem Cell Divisions from Paired Daughter Cell Expression Profiles Reveals Effects of Aging on Self-Renewal

Matthias Lütolf, Aline Roch

Changes in stem cell activity may underpin aging. However, these changes are not completely understood. Here, we combined single-cell profiling with machine learning and in vivo functional studies to explore how hematopoietic stem cell (HSC) divisions patt ...

CELL PRESS2020

Specification of haematopoietic stem cell fate via modulation of mitochondrial activity

Johan Auwerx, Matthias Lütolf, Aline Roch, Olaia Maria Naveiras Torres-Quiroga, Mukul Girotra, Nicola Vannini, Vasco Ledebur de Antas de Campos, Gennady Nikitin, Sonja Giger

Haematopoietic stem cells (HSCs) differ from their committed progeny by relying primarily on anaerobic glycolysis rather than mitochondrial oxidative phosphorylation for energy production. However, whether this change in the metabolic program is the cause ...

Nature Publishing Group2016

Brief Report: Single-Cell Analysis Reveals Cell Division-Independent Emergence of Megakaryocytes From Phenotypic Hematopoietic Stem Cells

Matthias Lütolf, Aline Roch, Vincent Trachsel

Despite increasingly stringent methods to isolate hematopoietic stem cells (HSCs), considerable heterogeneity remains in terms of their long-term self-renewal and differentiation potential. Recently, the existence of long-lived, self-renewing, myeloid-rest ...

Wiley-Blackwell2015

Mitochondrial Activity Determines Hematopoietic Stem Cell Fate Decisions

Matthias Lütolf, Johan Auwerx, Aline Roch, Mukul Girotra, Olaia Maria Naveiras Torres-Quiroga, Nicola Vannini, Vasco Ledebur de Antas de Campos

Amer Soc Hematology2014

In vitro Fate Mapping of Single Hematopoietic Stem Cells

Aline Roch

The in vitro expansion of hematopoietic stem cells (HSC) for clinical applications is hampered by a rapid loss of HSC blood reconstitution capability in culture. While these rare cells can be stimulated to massively proliferate, cell divisions mostly resul ...

EPFL2014

In Vivo Modulation of Mitochondrial Activity Determines HSC Engraftment and Post-Transplant Survival in Mice

Matthias Lütolf, Johan Auwerx, Aline Roch, Mukul Girotra, Olaia Maria Naveiras Torres-Quiroga, Nicola Vannini, Richardus Houtkooper, Eija Riitta Pirinen, Vasco Ledebur de Antas de Campos

Amer Soc Hematology2012

Dynamic interaction networks in a hierarchically organized tissue

Aline Roch

Intercellular (between cell) communication networks maintain homeostasis and coordinate regenerative and developmental cues in multicellular organisms. Despite the importance of intercellular networks in stem cell biology, their rules, structure and molecu ...

2010