Publications de Anja Kunze | EPFL Graph Search

Astrocyte–neuron co-culture on microchips based on the model of SOD mutation to mimic ALS

Philippe Renaud, Pierre Magistretti, Patrick Aebischer, Sylvain Eric Lengacher, Anja Kunze, Elisabeth Anne Dirren

Amyotrophic lateral sclerosis (ALS) is the most common motor neuron disease. ALS is believed to be a non-cell autonomous condition, as other cell types, including astrocytes, have been implicated in disease pathogenesis. Hence, to facilitate the developmen ...

Royal Soc Chemistry2013

Micro-engineering the Cerebral Cortical Cell Niche : A new Cell Culture Tool for Neuroscience Research

Anja Kunze

A major problem in traditional cell culture methods, such as Petri dishes and culture flasks, is the very simplified artificial environment around the cells. Traditional cell culture methods lack features of the native cell niche, such as gradients and cel ...

EPFL2012

Synergistic NGF/B27 Gradients Position Synapses Heterogeneously in 3D Micropatterned Neural Cultures

Philippe Renaud, Dominique Zosso, Ana Valero, Anja Kunze

Native functional brain circuits show different numbers of synapses (synaptic densities) in the cerebral cortex. Until now, different synaptic densities could not be studied in vitro using current cell culture methods for primary neurons. Herein, we presen ...

Public Library of Science2011

Micropatterning neural cell cultures in 3D with a multi-layered scaffold

Philippe Renaud, Michele Giugliano, Ana Valero, Anja Kunze

Cortical neurons, in their native state, are organized in six different cell layers; and the thickness of the cell layer ranges from 0.12 mm to 0.4 mm. The structure of cell layers plays an important role in neurodegenerative diseases or corticogenesis. We ...

2011

Synapse distribution in a 2D-3D micropatterned neural cell culture

Philippe Renaud, Anja Kunze

2011

Co-pathological states of tau proteins in a 3D micropatterned neural cell culture

Philippe Renaud, Robert Meissner, Anja Kunze, Serena Brando

In vitro studies of Alzheimer’s disease are based on homogenously seeded neural cells. These studies reveal molecular pathway mechanism related to neurite degeneration, but they cannot analyze interactions between diseased and healthy cells. Furthermore, t ...

2011

Co-Pathological Connected Primary Neurons in a Microfluidic Device for Alzheimer Studies

Philippe Renaud, Robert Meissner, Anja Kunze, Serena Brando

This communication presents a novel experi- mental model for Alzheimer studies, where connected pri- mary neurons were set into subtend, co-pathological states. Cortical neurons were cultured in two separated cell com- partments in a microfluidic device. A ...

Wiley-Blackwell2011

Neurite guidance through 3D hydrogel layers in a microfluidic environment

Philippe Renaud, Robert Meissner, Anja Kunze

Neuronal cell culture models in vitro are often restricted to 2D surfaces. Engineering the complexity of the neuronal microenvironment in microfluidic systems can help to generate more tissue like cultures. We have developed a new neuronal cell culturing s ...

2010

Sequential build-up of hydrogel environments around single cells

Philippe Renaud, Thomas Michael Braschler, Ana Valero, Anja Kunze, Ludovica Colella, Joël Theytaz, Grazia Loche

Controlling alginate gel formation by diffusion of Ca ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It gives the possibility of exposing cells under biocompatible condi ...

2009

A virtual valve for smooth contamination-free flow switching

Philippe Renaud, Thomas Michael Braschler, Harald Van Lintel, Nicolas Demierre, Anja Kunze, Mario Schlund, Raphaël Tornay, Joël Theytaz, Grazia Loche

We present a channel geometry that allows for clean switching between different inlets of a microchip without any contamination of the inlets or the downstream flow. We drive this virtual valve with a pneumatic pressure setup that minimizes disturbance of ...

2007