Publications de Debora Keller | EPFL Graph Search

Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells

Pierre Gönczy, Suliana Manley, Romain Wyss, Debora Keller

SAS-6 proteins are thought to impart the ninefold symmetry of centrioles, but the mechanisms by which their assembly occurs within cells remain elusive. In this paper, we provide evidence that the N-terminal, coiled-coil, and C-terminal domains of HsSAS-6 ...

Rockefeller University Press2014

Simple buffers for 3D STORM microscopy

Pierre Gönczy, Suliana Manley, Debora Keller, Vinoth Edal Joseph Sundar Rajan

3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging: making dye molecu ...

Optical Soc Amer2013

Resolution Doubling in 3D-STORM Imaging through Improved Buffers

Pierre Gönczy, Suliana Manley, Debora Keller

Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolution ...

Public Library of Science2013

Towards understanding HsSAS-6, a key player in centriole duplication

Debora Keller

The centrosome is the cell’s main microtubule organizing center. In order to assemble a proper bipolar spindle in mitosis, the cell has to duplicate its inherited single centrosome. During centrosome duplication, a single procentriole assembles approximate ...

EPFL2012

The SCF-FBXW5 E3-ubiquitin ligase is regulated by PLK4 and targets HsSAS-6 to control centrosome duplication

Pierre Gönczy, Debora Keller

Deregulated centrosome duplication can result in genetic instability and contribute to tumorigenesis(1,2). Here, we show that centrosome duplication is regulated by the activity of an E3-ubiquitin ligase that employs the F-box protein FBXW5 (ref. 3) as its ...

Nature Publishing Group2011

Structural Basis of the 9-Fold Symmetry of Centrioles

Pierre Gönczy, Debora Keller

The centriole, and the related basal body, is an ancient organelle characterized by a universal 9-fold radial symmetry and is critical for generating cilia, flagella, and centrosomes. The mechanisms directing centriole formation are incompletely understood ...

2011

Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL

Pierre Gönczy, Debora Keller, Petr Strnad, Fernando Romero Balestra, Gregor Kohlmaier

Patients with MCPH (autosomal recessive primary microcephaly) exhibit impaired brain development, presumably due to the compromised function of neuronal progenitors. Seven MCPH loci have been identified, including one that encodes centrosome protein 4.1 as ...

Company of Biologists2011

PP2A Phosphatase Acts upon SAS-5 to Ensure Centriole Formation in C. elegans Embryos

Pierre Gönczy, Debora Keller

Centrosome duplication occurs once per cell cycle and ensures that the two resulting centrosomes assemble a bipolar mitotic spindle. Centriole formation is fundamental for centrosome duplication. In Caenorhabditis elegans, the evolutionarily conserved prot ...

Cell Press - Elsevier2011

Youth careers in life sciences in Europe A white paper

Debora Keller

Elsevier2010

Youth in action for a better career-Introducing a White Paper on Life Sciences Careers in Europe

Debora Keller

2010