Publications de David Vincent Bonzon

Impedance-Based Single-Cell Pipetting

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, Jean-Baptiste Bureau, Georges Muller, Nicolas Jean Bernard Beuchat, Nicolas Uffer

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here ...

SAGE PUBLICATIONS INC2020

Traceable Impedance-Based Dispensing and Cloning of Living Single Cells

Philippe Renaud, Yann Barrandon, Ariane Rochat, David Vincent Bonzon, Stéphanie Claudinot, Georges Muller

Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using ...

2020

Micropipette calibration by differential pressure measurements

Philippe Renaud, David Vincent Bonzon

The pipetting of small volumes, typically ranging between a few and one thousand microliters (mu l), is widely used in many laboratories and essentially relies on air displacement to aspirate and dispense the liquid contained in a small plastic tip. ...

IOP PUBLISHING LTD2019

Tip connector for fluidic and electrical connection

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, David Forchelet, Jean-Baptiste Bureau, Georges Muller, Nicolas Uffer

A connector for providing both a fluidic and electrical connection is disclosed, said connector having a proximal end, a distal end and an elongated body in between, characterized in that the elongated body has an inner cavity spanning throughout its lengt ...

2018

Microfluidic sensor and method for obtaining such a sensor

Philippe Renaud, Luc Gaëtan Aeberli, Thomas Michael Braschler, David Vincent Bonzon, Marc Lany, Georges Muller, Jonas Choppe, Niklas Van Neyghem

The invention describes a method for producing hybrid microelectronic/microfluidic sensors at industrial scale. The method is characterized in that it comprises the following steps for obtaining said microfluidic channel: a) a first lamination step of a dr ...

2018

Impedance Spectroscopy for Single Cell Analysis and Dispensing

David Vincent Bonzon

Flow cytometry is an essential tool in biology and medicine with applications in immunodeficiency diagnostic, immunosurveillence, organ transplantation in addition to its basic research usage. Before the widespread use of the Fluorescent-Activated Cell Sor ...

EPFL2017

Devices, systems and methods for dispensing and analysing particles

Philippe Renaud, Yann Barrandon, Jean-Baptiste Bureau, David Vincent Bonzon, Steve Béguin

The present invention relates to a pipette tip comprising a thin holed membrane at its distal end, which is designed to be adapted with a system comprising at least an impedance analyser and a fluidic actuator to perform the dispensing and analysis of part ...

2016

Observation device with optical compensation

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, David Forchelet, Steve Béguin

The invention concerns an observation device such as cell culture wells comprising an optical element such as a lens or a filter, or even combinations thereof, for compensating an optical effect induced on a sample contained in the observation device and i ...

2016

Sensing Tip with Electrical Impedance Sensor

Philippe Renaud, David Vincent Bonzon, Yann Barrandon

The invention relates to a sensing tip (8) comprising a conventional tip (1), such as a pipette tip, which is essentially made of a cavity which communicates with the external environment through an aperture (3) located at the tip distal end; the sensing t ...

2016

Biomimetic Pieris rapae’s Nanostructure and Its Use as a Simple Sucrose Sensor

Philippe Renaud, David Vincent Bonzon, Rodrigo Martinez Duarte

iomimetics often provides efficient ways to create a product incorporating novel properties. Here we present the replication of the Pieris rapae butterfly optical structure. This butterfly has white wings with black spots. The white coloration is produced ...

MDPI2014