Publications de Andrija Finka | EPFL Graph Search

Experimental Milestones in the Discovery of Molecular Chaperones as Polypeptide Unfolding Enzymes

Molecular chaperones control the cellular folding, assembly, unfolding, disassembly, translocation, activation, inactivation, disaggregation, and degradation of proteins. In 1989, groundbreaking experiments demonstrated that a purified chaperone can bind a ...

Annual Reviews2016

Quantitative proteomics of heat-treated human cells show an across-the-board mild depletion of housekeeping proteins to massively accumulate few HSPs

Paolo De Los Rios, Andrija Finka, Pierre Goloubinoff, Vishal Sood

Classic semiquantitative proteomic methods have shown that all organisms respond to a mild heat shock by an apparent massive accumulation of a small set of proteins, named heat-shock proteins (HSPs) and a concomitant slowing down in the synthesis of the ot ...

Springer2015

A Novel Method for the Encapsulation of Biomolecules into Polymersomes via Direct Hydration

Jeffrey Alan Hubbell, Davide Demurtas, Conlin O'Neil, Andrija Finka

One of the major engineering challenges for the implementation of block copolymer vesicles, or polymersomes, as therapeutic drug carriers is obtaining high encapsulation efficiencies for biomolecules. Here we present it novel method for encapsulation of pr ...

2009

Magnetic, paramagnetic and/or superparamagnetic nanoparticles

Heinrich Hofmann, Alke Fink, Benedikt Steitz, Andrija Finka, Jatuporn Salaklang

The present invention relates to nanoparticles having a mean diameter of < 500nm and comprising, at their surface, a selected material. The nanoparticles are taken up by cells under physiological conditions and can be used to isolate interaction partners o ...

2009

Novel 7-mer peptide mediates nuclear targeting in MCF7 breast cancer cells

Jeffrey Alan Hubbell, Andrija Finka

2009

Dielectrophoresis-based particle exchanger for the manipulation and surface functionalization of particles

Philippe Renaud, Heinrich Hofmann, Jeffrey Alan Hubbell, Thomas Michael Braschler, Nicolas Demierre, Benedikt Steitz, Raphaël Tornay, Andrija Finka

We present a microfluidic device where micro- and nanoparticles can be continuously functionalized in flow. This device relies on an element called particle exchanger, which allows for particles to be taken from one medium and exposed to some reagent while ...

2008

Effect of cell media on polymer coated superparamagnetic iron oxide nanoparticles (SPIONs): Colloidal stability, cytotoxicity, and cellular uptake studies

Heinrich Hofmann, Alke Fink, Benedikt Steitz, Andrija Finka, Jatuporn Salaklang

The influence of the composition of the polymer coated polyvinyl alcohol (PVA), vinyl alcohol/vinyl amine copolymer (A-PVA) and polyethylenimine (PEI) coated superparamagnetic iron oxide nanoparticles (SPIONs) on the colloidal stability, cytotoxicity and c ...

2008

Superparamagnetic Nanoparticles as a Powerful Systems Biology. Characterization Tool in the Physiological Context

Heinrich Hofmann, Jeffrey Alan Hubbell, Alke Fink, Benedikt Steitz, Marc Moniatte, Conlin O'Neil, Andrija Finka, Jatuporn Salaklang

Recently, functionalized superparamagnetic iron oxide nanoparticles (SPIONs) have been utilized for protein separation and therapeutic delivery of DNA and drugs. The development of new methods and tools for the targeting and identification of specific biom ...

Wiley-Blackwell2008

Fixed bed reactor for solid phase surface derivatization of superparamagnetic nanoparticles

Heinrich Hofmann, Alke Fink, Benedikt Steitz, Andrija Finka, Jatuporn Salaklang

The functionalization of nanoparticles is conditio sine qua non in studies of specific interaction with a biological target. Often, their biological functionality is achieved by covalent binding of bioactive molecules on a preexisting single surface coatin ...

2007