Jonas Milani

Cette personne n’est plus à l’EPFL

Publications associées (29)

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Hyperpolarized NMR of plant and cancer cell extracts at natural abundance

Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Basile Vuichoud

Natural abundance C-13 NMR spectra of biological extracts are recorded in a single scan provided that the samples are hyperpolarized by dissolution dynamic nuclear polarization combined with cross polarization. Heteronuclear 2D correlation spectra of hyper ...

Royal Society of Chemistry2015

Hyperpolarization of Deuterated Metabolites via Remote Cross-Polarization and Dissolution Dynamic Nuclear Polarization

Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Basile Vuichoud

In deuterated molecules such as [1-C-13]pyruvate-d(3), the nuclear spin polarization of C-13 nuclei can be enhanced by combining Hartmann-Hahn cross-polarization (CP) at low temperatures (1.2 K) with dissolution dynamic nuclear polarization (D-DNP). The po ...

American Chemical Society2014

Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization

Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Daniel Abergel

Dissolution dynamic nuclear polarization (D-DNP) experiments enabled us to study the kinetics of the enzymatic phosphorylation reaction of glucose to form glucose-6-phosphate (G6P) by hexokinase (HK), with or without the presence of an excess of G6P, which ...

2014

Drug Screening Boosted by Hyperpolarized Long-Lived States in NMR

Geoffrey Bodenhausen, Sandrine Gerber, Aurélien Bornet, Jonas Milani, Sami Jannin, Basile Vuichoud, Solène Marcelle Françoise Marie Passemard, Nicola Salvi, Daniele Mammoli, Roberto Buratto

Transverse and longitudinal relaxation times (T11 and T1) have been widely exploited in NMR to probe the binding of ligands and putative drugs to target proteins. We have shown recently that long-lived states (LLS) can be more sensitive to ligand binding. ...

Wiley-Blackwell2014

Cross polarization from H-1 to quadrupolar Li-6 nuclei for dissolution DNP

Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Basile Vuichoud, Angel Joaquin Perez Linde

Cross polarization from protons to quadrupolar Li-6 nuclei is combined with dynamic nuclear polarization of protons at 1.2 K and 6.7 T using TEMPOL as a polarizing agent followed by rapid dissolution. Compared to direct Li-6 DNP without cross-polarization, ...

Royal Society of Chemistry2014

Long-Lived States of Magnetically Equivalent Spins Populated by Dissolution-DNP and Revealed by Enzymatic Reactions

Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Basile Vuichoud, Daniele Mammoli, Xiao Ji

Hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) offers a way of enhancing NMR signals by up to five orders of magnitude in metabolites and other small molecules. Nevertheless, the lifetime of hyperpolarization is inexorably limited, a ...

Wiley-Blackwell2014

Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization

David Lyndon Emsley, Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Basile Vuichoud, David Benjamin Roger Antoine Gajan

Hyperpolarization of substrates for magnetic resonance spectroscopy (MRS) and imaging (MRI) by dissolution dynamic nuclear polarization (D-DNP) usually involves saturating the ESR transitions of polarizing agents (PAs; e.g., persistent radicals embedded in ...

National Academy of Sciences2014

Microwave frequency modulation to enhance Dissolution Dynamic Nuclear Polarization Dedicated to To Martial Rey, as a token of appreciation

Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Basile Vuichoud, Angel Joaquin Perez Linde

Hyperpolarization by Dissolution Dynamic Nuclear Polarization is usually achieved by monochromatic microwave irradiation of the ESR spectrum of free radicals embedded in glasses at 1.2 K and 3.35 T. Hovav et al. (2014) have recently shown that by using fre ...

Elsevier2014

Dynamic Nuclear Polarization and Other Magnetic Ideas at EPFL

Geoffrey Bodenhausen, Aurélien Bornet, Pascal Miéville, Sami Jannin, Jonas Milani, Diego Carnevale, Simone Ulzega, Marc Anthony Caporini, Nicola Salvi, Daniele Mammoli

Although nuclear magnetic resonance (NMR) can provide a wealth of information, it often suffers from a lack of sensitivity. Dynamic Nuclear Polarization (DNP) provides a way to increase the polarization and hence the signal intensities in NMR spectra by tr ...

2012

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Microwave frequency modulation to enhance Dissolution Dynamic Nuclear Polarization Dedicated to To Martial Rey, as a token of appreciation

Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Basile Vuichoud, Angel Joaquin Perez Linde

Elsevier2014