Personne
Etienne Shaffer
Cette personne n’est plus à l’EPFL
Publications associées (18)
Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening
Gerardo Turcatti, Benjamin Rappaz, Etienne Shaffer
Digital Holographic Microscopy (DHM) is a label-free imaging technique allowing visualization of transparent cells with classical imaging cell culture plates. The quantitative DHM phase contrast image provided is related both to the intracellular refractiv ...
2014Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy
Pierre Magistretti, Christian Depeursinge, Gerardo Turcatti, Benjamin Rappaz, Marc Chambon, Jonas Kühn, Etienne Shaffer, Jérôme Parent, Julien Mena
Abstract We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content ...
Mary Ann Liebert2013Single-shot, simultaneous incoherent and holographic microscopy
Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer
In this work, we demonstrate single-shot, simultaneous recording and subsequent retrieval of one incoherent and two holographic (intensity and phase) images from the same camera frame. Demultiplexing of incoherent and holographic signals in the spatial fre ...
Wiley-Blackwell2012Holographic second harmonic phase microscopy of collagen fibers
In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...
2011Holographic Second Harmonic Generation Microscopy
This dissertation is dedicated to the application of digital holography methods to second harmonic generation microscopy, and to the development of the resulting imaging technique: holographic second harmonic generation microscopy. Second harmonic generati ...
EPFL2011Second harmonic phase microscopy of collagen fibers
Christian Depeursinge, Pierre Marquet, Etienne Shaffer
In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...
SPIE2011Holographic second harmonic generation (SHG) microscopy
Christian Depeursinge, Pierre Marquet, Etienne Shaffer
The coherent nature of second harmonic generation (SHG) makes possible retrieval of both its amplitude and its phase, by use of a proper phase-sensitive imaging technique such as digital holographic SHG microscopy. In addition to retrieving both amplitude ...
2011Second harmonic phase microscopy of collagen fibers
Christian Depeursinge, Pierre Marquet, Etienne Shaffer
In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...
Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2011Digital holography for second harmonic microscopy
Christian Depeursinge, Etienne Shaffer
Quantitative phase images make digital holographic microscopy (DHM) an excellent instrument for metrological, but also for biological applications, where it can reveal deformations and morphological details at ultrahigh resolution in the order of a few nan ...
SPIE2010Real time, nanometric 3D-tracking of nanoparticles made possible by second harmonic generation digital holographic microscopy
Christian Depeursinge, Pierre Marquet, Etienne Shaffer
In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...
Optical Society of America2010