Publications de Mohammad Reza Pourhaghighi

Porous silica enhanced proteolysis during Off-Gel separation for efficient protein identification

Hubert Girault, Liang Qiao, Mohammad Reza Pourhaghighi

An advanced approach is developed in this work for simultaneous on-line separation and digestion of proteins by combining the Off-Gel isoelectric focusing (IEF) and enzymatic nanoreactor enhanced proteolysis. The nanoreactor was prepared by preloading tryp ...

Elsevier2015

A microchip emitter for solid phase extraction – gradient elution – mass spectrometry

Hubert Girault, Natalia Gasilova, Liang Qiao, Dmitry Momotenko, Mohammad Reza Pourhaghighi

A microchip electrospray emitter with a magnetic bead trap has been designed for solid phase extraction-gradient elution-mass spectrometry (SPE-GEMS). The goal of this method is the detection of analytes at low concentrations and it is here demonstrated us ...

American Chemical Society2013

Development of Electrokinetically Based Two-Dimensional Separation Methodologies for Proteomic Studies

Mohammad Reza Pourhaghighi

Proteomic studies require analytical methods capable of separating, identifying and quantifying thousands of proteins. Consequently, developing separation methodologies with high resolving power necessary to separate complex protein samples prior to protei ...

EPFL2012

High-sensitive protein analysis by FESI-CEMALDI-MS

Hubert Girault, Mohammad Reza Pourhaghighi

Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iont ...

Wiley-Blackwell2011

LEPA: From Proteomics to Energy Conversion

Hubert Girault, Liang Qiao, Fernando Cortes Salazar, Manuel Alejandro Méndez Agudelo, Anne-Laure Gassner, Mohammad Reza Pourhaghighi

This review article summarizes the different fields of research at the Laboratoire d'Electrochimie Physique et Analytique at the Ecole Polytechnique Federale de Lausanne. The research areas covered include charge transfer reactions at soft interfaces, bio- ...

2011