Publications de Helen Mary Rowe

A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819

Didier Trono, Gabriela Ecco, Helen Mary Rowe

Mammalian genomes are a battleground for genetic conflict between repetitive elements and KRAB-zinc finger proteins (KZFPs). We asked whether KZFPs can regulate cell fate by using ZFP819, which targets a satellite DNA array, ZP3AR. ZP3AR coats megabase reg ...

2022

Transposable Elements and Their KRAB-ZFP Controllers Regulate Gene Expression in Adult Tissues

Didier Trono, Priscilla Turelli, Sandra Eloise Kjeldsen, Gabriela Ecco, Julien Léonard Duc, Marco Cassano, Annamaria Kauzlaric, Andrea Coluccio, Michaël Imbeault, Helen Mary Rowe

KRAB-containing zinc finger proteins (KRAB-ZFPs) are early embryonic controllers of transposable elements (TEs), which they repress with their cofactor KAP1 through histone and DNA methylation, a process thought to result in irreversible silencing. Using a ...

Cell Press - Elsevier2016

A Large-Scale Functional Screen to Identify Epigenetic Repressors of Retrotransposon Expression

Didier Trono, Gabriela Ecco, Helen Mary Rowe

Deposition of epigenetic marks is an important layer of the transcriptional control of retrotransposons, especially during early embryogenesis. Krüppel-associated box domain zinc finger proteins (KRAB-ZFPs) are one of the largest families of transcription ...

2016

Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency

Didier Trono, Priscilla Turelli, Bastien Mangeat, Evaristo Jose Planet Letschert, Gabriela Ecco, Marc Samuel Friedli, Adamantia Kapopoulou, Benjamin Rauwel, Helen Mary Rowe, Carmen Maria Unzu Ezquerro

Endogenous retroelements (EREs) account for about half of the mouse or human genome, and their potential as insertional mutagens and transcriptional perturbators is suppressed by early embryonic epigenetic silencing. Here, we asked how ERE control is maint ...

Cold Spring Harbor Lab Press, Publications Dept2014

Global and stage specific patterns of krüppel-associated-box zinc finger protein gene expression in murine early embryonic cells

Didier Trono, Bart Deplancke, Adamantia Kapopoulou, Andrea Corsinotti, Michaël Imbeault, Yoann Mouscaz, Carine Delattre-Gubelmann, Helen Mary Rowe

Highly coordinated transcription networks orchestrate the self-renewal of pluripotent stem cell and the earliest steps of mammalian development. KRAB-containing zinc finger proteins represent the largest group of transcription factors encoded by the genome ...

Public Library of Science2013

De novo DNA methylation of endogenous retroviruses is shaped by KRAB-ZFPs/KAP1 and ESET

Didier Trono, Sandra Eloise Kjeldsen, Sonia Verp, Marc Samuel Friedli, Helen Mary Rowe

Endogenous retroviruses (ERVs) undergo de novo DNA methylation during the first few days of mammalian embryogenesis, although the factors that control the targeting of this process are largely unknown. We asked whether KAP1 (KRAB-associated protein 1) is i ...

Company Of Biologists Ltd2013

KAP1 controls endogenous retroviruses in embryonic stem cells

Didier Trono, Daniel Constam, Séverine Reynard, Jacques Rougemont, Sonia Verp, Hillary Layard-Liesching, Helen Mary Rowe, Johan Jakobsson, Pierre Maillard

More than forty per cent of the mammalian genome is derived from retroelements, of which about one-quarter are endogenous retroviruses (ERVs). Some are still active, notably in mice the highly polymorphic early transposon (ETn)/MusD and intracisternal A-ty ...

Nature Publishing Group2010