Personne
Neeraj Dhar
Publications associées (45)
Investigating the composition and recruitment of the mycobacterial ImuA'-ImuB-DnaE2 mutasome
John McKinney, Neeraj Dhar, Zela Alexandria-Mae Martin
A DNA damage-inducible mutagenic gene cassette has been implicated in the emergence of drug resistance in Mycobacterium tuberculosis during anti-tuberculosis (TB) chemotherapy. However, the molecular composition and operation of the encoded 'mycobacterial ...
Cambridge2023Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection
John McKinney, Neeraj Dhar, Vivek Vijay Thacker, François Signorino-Gelo, Kunal Sharma, Thomas Marie Simonet
Uropathogenic Escherichia coli (UPEC) proliferate within superficial bladder umbrella cells to form intracellular bacterial communities (IBCs) during early stages of urinary tract infections. However, the dynamic responses of IBCs to host stresses and anti ...
2021Revealing Antibiotic Tolerance of the Mycobacterium smegmatis Xanthine/Uracil Permease Mutant Using Microfluidics and Single-Cell Analysis
John McKinney, Neeraj Dhar, Meltem Elitas
To reveal rare phenotypes in bacterial populations, conventional microbiology tools should be advanced to generate rapid, quantitative, accurate, and high-throughput data. The main drawbacks of widely used traditional methods for antibiotic studies include ...
MDPI2021Bacteria: Driving polar growth
Profiling the phenotype of 200,000 mutants revealed a new cofactor that may help a group of rod-shaped bacteria elongate and grow. ...
2020Preexisting variation in DNA damage response predicts the fate of single mycobacteria under stress
John McKinney, Neeraj Dhar, Giulia Manina, Lalit Kumar Singh
Clonal microbial populations are inherently heterogeneous, and this diversification is often considered as an adaptation strategy. In clinical infections, phenotypic diversity is found to be associated with drug tolerance, which in turn could evolve into g ...
2019Computational Analysis of the Mutual Constraints between Single‐Cell Growth and Division Control Models
John McKinney, Neeraj Dhar, Ambroise Roger Vuaridel
Three models of division control are proposed to achieve cell size homeostasis: sizer, timer, and adder. However, few published studies of division control take into account the dynamics of single‐cell growth and most assume that single‐cell growth is expo ...
2019Fluorescent Benzothiazinone Analogues Efficiently and Selectively Label Dpre1 in Mycobacteria and Actinobacteria
Paul Joseph Dyson, Stewart Cole, John McKinney, Neeraj Dhar, Thierry Laroche, Jérémie Piton, Joao Pedro De Almeida Neres, Raphael Christopher Sommer, Raju Mukherjee
Benzothiazinones (BTZ) are highly potent bactericidal inhibitors of mycobacteria and the lead compound, BTZ043, and the optimized drug candidate, PBTZ169, have potential for the treatment of tuberculosis. Here, we exploited the tractability of the BTZ scaf ...
2018An Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase is required for mycobacterial cell division
Neeraj Dhar, Ashima Bhaskar, Dong Li
Mycobacteria possess a multi-layered cell wall that requires extensive remodelling during cell division. We investigated the role of an amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase, a peptidoglycan remodelling enzyme implicated in cell div ...
Nature Publishing Group2017The Inosine Monophosphate Dehydrogenase, GuaB2, Is a Vulnerable New Bactericidal Drug Target for Tuberculosis
Stewart Cole, John McKinney, Neeraj Dhar, Claudia Sala, Ruben Hartkoorn, Anthony Vocat, Andréanne Lupien, Raphael Christopher Sommer
VCC234718, a molecule with growth inhibitory activity against Mycobacterium tuberculosis (Mtb), was identified by phenotypic screening of a 15344-compound library. Sequencing of a VCC234718-resistant mutant identified a Y487C substitution in the inosine mo ...
Amer Chemical Soc2017Division site selection linked to inherited cell surface wave troughs in mycobacteria
Georg Fantner, John McKinney, Neeraj Dhar, Adrian Pascal Nievergelt, Pascal Damian Odermatt, Haig Alexander Eskandarian, Mélanie Thérèse Marie Hannebelle, Joëlle Xiao Yuan Ven
Cell division is tightly controlled in space and time to maintain cell size and ploidy within narrow bounds. In bacteria, the canonical Minicell (Min) and nucleoid occlusion (Noc) systems together ensure that division is restricted to midcell after complet ...
2017