Publications de Ata Tuna Ciftlik

Computer-Based Intensity Measurement Assists Pathologists in Scoring Phosphatase and Tensin Homolog Immunohistochemistry - Clinical Associations in NSCLC Patients of the European Thoracic Oncology Platform Lungscape Cohort

Ata Tuna Ciftlik, Thomas Geiger, Ruben Casanova

Introduction: Phosphatase and tensin homolog (PTEN) loss is frequently observed in NSCLC and associated with both phosphoinositide 3-kinase activation and tumoral immunosuppression. PTEN immunohistochemistry is a valuable readout, but lacks standardized st ...

2018

Microfluidic network device

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Pierre Joris

Microfluidic network device (2) configured to supply reagents to a biological tissue sampling device (1), comprising a plurality of microfluidic inlet channels (12) connected to respective sources of said reagents, at least one common outlet channel (22), ...

2017

Method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Déborah Heintze

The invention relates to a method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof. In particular, the invention relates to a method which is a useful tool for use in the field of diagnosis, prevention and/or treat ...

2017

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Maryse Fiche, Déborah Heintze

Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...

Nature Research2016

Programming and use of Parylene C fluorescence as a quantitative on-chip reference

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Joan Teixidor

A large number of lab-on-a-chip applications use fluorescence for quantifying biological entities. In such applications, incorporation of a stable on-chip fluorescent reference source would be highly desirable in order to compensate for instrumental parame ...

Royal Soc Chemistry2014

Fabrication of Biocompatible Monolithic Microchannels with High Pressure-Resistance Using Direct Polymerization of PEG-Modified PMMA

Martinus Gijs, Ata Tuna Ciftlik, Elena Bianchi

Withstanding high pressures in polymeric microchannels is an important requirement for many biological applications. Here, a simple direct polymerization through a polyester photomask is applied to obtain monolithic polyethylene glycol (PEG)-modified poly( ...

Wiley-Blackwell2014

Sample processing device with detachable slide

Martinus Gijs, Ata Tuna Ciftlik

A biological and chemical sample processing device that b. comprises a high pressure-resistant, shallow and wide area microfluidic chamber having at least one wall formed by a detachable slide containing samples such as immobilized entities, biological sam ...

2013

Characterization of short incubation time effects on chromogen signal obtained by HER2-expressing breast carcinomas using microfluidic immunohistochemistry

Martinus Gijs, Ata Tuna Ciftlik, Hans-Anton Lehr

2013

Opportunities for high-pressure microfluidics in biomedical applications

Ata Tuna Ciftlik

Pressure lies at the basis of operation of most microfluidic systems, and is a determinant factor in the extent of miniaturization and in limiting the throughput and time constant of the microfluidic assays. Despite the apparent importance given to fluidic ...

EPFL2013

Microfluidic processor allows rapid HER2 immunohistochemistry of breast carcinomas and significantly reduces ambiguous (2+) read-outs

Martinus Gijs, Ata Tuna Ciftlik, Hans-Anton Lehr

Biomarker analysis is playing an essential role in cancer diagnosis, prognosis, and prediction. Quantitative assessment of immunohistochemical biomarker expression on tumor tissues is of clinical relevance when deciding targeted treatments for cancer patie ...

National Academy of Sciences2013