Publications de Niccolo Piacentini

Toward a Physiologically Relevant 3D Helicoidal-Oriented Cardiac Model: Simultaneous Application of Mechanical Stimulation and Surface Topography

Philippe Renaud, Niccolo Piacentini, Fatemeh Navaee

Myocardium consists of cardiac cells that interact with their environment through physical, biochemical, and electrical stimulations. The physiology, function, and metabolism of cardiac tissue are affected by this dynamic structure. Within the myocardium, ...

MDPI2023

Additive manufacturing of hierarchical injectable scaffolds for tissue engineering

Philippe Renaud, Niccolo Piacentini, Thomas Michael Braschler

We present a 3D-printing technology allowing free-form fabrication of centimetre-scale injectable structures for minimally invasive delivery. They result from the combination of 3D printing onto a cryogenic substrate and optimisation of carboxymethylcellul ...

2018

Multimaterial Nanoporous Membranes Shaped through High Aspect-Ratio Sacrificial Silicon Nanostructures

Philippe Renaud, Arnaud Bertsch, Niccolo Piacentini, Stefano Silvio Giovanni Varricchio

We present an innovative fabrication method for solid-state nanoporous membranes based on the casting of sacrificial silicon nanostructures. The process allows the individual definition of geometry and placement of each nanopore through e-beam lithography ...

Amer Chemical Soc2017

Fabrication of nanoporous membranes through silicon templates : two different approaches

Philippe Renaud, Arnaud Bertsch, Niccolo Piacentini, Stefano Silvio Giovanni Varricchio

We present here a novel approach to fabricate nanoporous membranes based on the use of sacrificial template structures to individually define pore parameters such as geometry, shape, and placement. Based on this rationale two different nano-fabrication met ...

2016

Design And Fabrication Of A Well-set, Cost-effective Device For Mechanical And Biochemical Stimulation In Cardiac Tissue Engineering Applications

Philippe Renaud, Fatemeh Navaee, Niccolo Piacentini

2016

Intercellular ultrafast Ca2+ wave in vascular smooth muscle cells: numerical and experimental study

Jean-Jacques Meister, Niccolo Piacentini, Franck Claude Raynaud, Jairo Camilo Quijano Perez

Vascular smooth muscle cells exhibit intercellular Ca2+ waves in response to local mechanical or KCl stimulation. Recently, a new type of intercellular Ca2+ wave was observed in vitro in a linear arrangement of smooth muscle cells. The intercellular wave w ...

Nature Research2016

Evaluating Tension in Actomyosin Bundles at the Cell Periphery

Jean-Jacques Meister, Niccolo Piacentini, Benoît Vianay, Céline Jeanne Labouesse

Cell Press2014

Ultra-soft cantilevers and 3-D micro-patterned substrates for contractile bundle tension measurement in living cells

Jean-Jacques Meister, Niccolo Piacentini, Chiara Gabella, Alexander Verkhovskiy, Benoît Vianay

Actin-myosin microfilament bundles or stress-fibers are the principal tension-generating structures in the cell. Their mechanical properties are critical for cell shape, motion, and interaction with other cells and extracellular matrix, but were so far dif ...

Royal Society of Chemistry2014

Contact Angle at the Leading Edge Controls Cell Protrusion Rate

László Forró, Jean-Jacques Meister, Niccolo Piacentini, Chiara Gabella, Alexander Verkhovskiy, Sylvia Jeney, Elena Bertseva, Alicia Carine Bornert

Plasma membrane tension and the pressure generated by actin polymerization are two antagonistic forces believed to define the protrusion rate at the leading edge of migrating cells [1-5]. Quantitatively, resistance to actin protrusion is a product of membr ...

Elsevier2014

Multiple-frequency impedance measurements in continuous flow for automated evaluation of yeast cell lysis

Philippe Renaud, Niccolo Piacentini, Willyan Hasenkamp Carreira

This paper reports on an electrical lysis device integrating multiple-frequency impedance measurements for automated evaluation of the lysis efficiency and counting of the lysed cells. Conventional techniques use chemical reagents to dissolve the cell memb ...

Elsevier2012