Publications de Suliana Manley

An Updated Inventory of Genes Essential for Oxidative Phosphorylation Identifies a Mitochondrial Origin in Familial Menie`re's Disease

Suliana Manley, Juan Cruz Landoni Martin

Mitochondrial disorders (MDs) are among the most common inborn errors of metabolism, and dysfunction in oxidative phosphorylation (OXPHOS) is a hallmark. Their complex mode of inheritance and diverse clinical presentations render the diagnosis of MDs chall ...

CELL PRESS2025

Resolution in super-resolution microscopy – facts, artifacts, technological advancements and biological applications

Aleksandra Radenovic, Suliana Manley

Super-resolution microscopy (SRM) has undeniable potential for scientific discovery, yet still presents many challenges that hinder its widespread adoption, including technical trade-offs between resolution, speed and photodamage, as well as limitations in ...

The Company of Biologists2025

Spotiflow: accurate and efficient spot detection for fluorescence microscopy with deep stereographic flow regression

Joachim Lingner, Suliana Manley, Gioele La Manno, Can Aztekin, Irina Khven, Eftychia Kyriacou, Albert Dominguez Mantes, Evangelia Skoufa, Martin Weigert, Georgios Tsissios

Identification of spot-like structures in large, noisy microscopy images is a crucial step for many life-science applications. Imaging-based spatial transcriptomics (iST), in particular, relies on the precise detection of millions of transcripts in low sig ...

2025

Live-cell imaging powered by computation

Suliana Manley, Florian Jug

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

Nature Portfolio2024

Mitochondrial Structure, Dynamics, and Physiology Light Microscopy to Disentangle the Network

Suliana Manley, Willi Leopold Stepp, Julius Hans Bernd Winter, Juan Cruz Landoni Martin, Tatjana Isabelle Kleele

Mitochondria serve as energetic and signaling hubs of the cell: This function results from the complex interplay between their structure, function, dynamics, interactions, and molecular organization. The ability to observe and quantify these properties oft ...

ANNUAL REVIEWS2024

Label-Free Imaging of DNA Interactions with 2D Materials

Aleksandra Radenovic, Suliana Manley, Jenny Sülzle, Wayne Yang Wen Wei, Nathan Cyprien Arsène Ronceray, Eveline Simone Mayner, Yuta Shimoda

Two-dimensional (2D) materials offer potential as substrates for biosensing devices, as their properties can be engineered to tune interactions between the surface and biomolecules. Yet, not many methods can measure these interactions in a liquid environme ...

2024

Surface passivation and functionalisation for mass photometry

Suliana Manley, Jenny Sülzle, Laila Abdelaziz Abdelmoniem Elfeky

Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecules. Consequently, single-particle imaging and tracking with the iSCAT-based method known as mass photometry (MP) is a growing area of study. However, establishing ...

Wiley2024

DNA-PAINT adaptors make for efficient multiplexing

Suliana Manley

Multiplexed super -resolution imaging offers a route to spatial proteomics; however, time -efficient mapping of many protein species has been challenging. Two recent works in Cell highlight SUM -PAINT and FLASHPAINT, methods that leverage adaptor DNA stran ...

CELL PRESS2024

Resolution in super-resolution microscopy — definition, trade-offs and perspectives

Aleksandra Radenovic, Suliana Manley

Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and pos ...

2024

Chromosome organization shapes replisome dynamics in Caulobacter crescentus

Suliana Manley, Chen Zhang, Laurent Casini

DNA replication in bacteria takes place on highly compacted chromosomes, where segregation, transcription, and repair must occur simultaneously. Within this dynamic environment, colocalization of sister replisomes has been observed in many bacterial specie ...

Nature Portfolio2024