Publications de Hui Yang | EPFL Graph Search

EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization

Determining the spatial organization and morphological characteristics of molecularly defined cell types is a major bottleneck for characterizing the architecture underpinning brain function. We developed Expansion Assisted Iterative Fluorescence In Situ H ...

CELL PRESS2021

Microsphere-based super-resolution scanning optical microscope

Martinus Gijs, Hui Yang, Gergely Huszka

High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with su ...

Optical Soc Amer2017

System for optical detection and imaging of sub-diffraction-limited nano-objects

Martinus Gijs, Matteo Cornaglia, Hui Yang

Nano-objects (8) with feature size smaller than the classical Abbe's diffraction limit can be optically detected via a microlens (4) array configured in a miniaturized microfluidic device. A hydrophilic microwell (3) array in a hydrophobic layer (2) is fir ...

2016

Super-Resolution Imaging of a Dielectric Microsphere Is Governed by the Waist of Its Photonic Nanojet

Martinus Gijs, Raphaël Etienne Jean Trouillon, Hui Yang, Gergely Huszka

Dielectric microspheres with appropriate refractive index can image objects with super-resolution, that is, with a precision well beyond the classical diffraction limit. A microsphere is also known to generate upon illumination a photonic nanojet, which is ...

American Chemical Society2016

Determination of sub-diffraction-limited organelles and biomolecules using microsphere lenses in combination with fluorescence microscopy

Johan Auwerx, Martinus Gijs, Norman Lionel Moullan, Hui Yang

We propose the use of a high-refractive index glass microsphere in combination with a conventional fluorescence microscope for imaging sub-cellular organelles and biomolecules. The microsphere is placed on a sample that is immersed in water, collects the n ...

Spie-Int Soc Optical Engineering2015

Photonic Nanojet Array for Fast Detection of Single Nanoparticles in a Flow

Martinus Gijs, Matteo Cornaglia, Hui Yang

We detect by optical microscopy Au and fluorescent nanopartides (NPs) during their motion in water based medium, using an array of dielectric microspheres that are patterned in a microwell array template. The Microspheres act as lenses focusing the light o ...

American Chemical Society2015

Study of constrained Brownian motion of nanoparticles near an interface using optical tweezers

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hui Yang

We demonstrate a method to determine the Brownian motion and the diffusion coefficient of a nanoparticle in water in a plane that is parallel to a solid boundary and as function of the distance normal to that boundary by using an optical tweezers instrumen ...

Spie-Int Soc Optical Engineering2015

Optical microscopy using a glass microsphere for metrology of sub-wavelength nanostructures

Martinus Gijs, Hui Yang

A technique that allows direct optical imaging of nanostructures and determines quantitatively geometric nanofeatures beyond the classical diffraction limit by using high-refractive index glass microspheres is introduced. The glass microsphere is put on a ...

Elsevier2015

Microsphere lenses for microfluidic bio-assays and for super-resolution imaging of biological structures

Hui Yang

EPFL2014

Super-Resolution Biological Microscopy Using Virtual Imaging by a Microsphere Nanoscope

Johan Auwerx, Martinus Gijs, Norman Lionel Moullan, Hui Yang

Wiley-Blackwell2014