Personne

Shiyu Chen

Cette personne n’est plus à l’EPFL

Publications associées (12)

Graph Chatbot

Chattez avec Graph Search

Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.

AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.

Connectez-vous pour utiliser Chat avec Graph Search

A Synthetic Factor XIIa Inhibitor Blocks Selectively Intrinsic Coagulation Initiation

Christian Heinis, Shiyu Chen, Alessandro Zorzi, Vanessa Baeriswyl, Luca Bologna

Coagulation factor XII (FXII) inhibitors are of interest for the study of the protease in the intrinsic coagulation pathway, for the suppression of contact activation in blood coagulation assays, and they have potential application in antithrombotic therap ...

American Chemical Society2015

Peptide ligands stabilized by small molecules

Christian Heinis, Florence Pojer, Alessandro Angelini, Shiyu Chen, Davide Bertoldo

Bicyclic peptides generated through directed evolution by using phage display offer an attractive ligand format for the development of therapeutics. Being nearly 100-fold smaller than antibodies, they promise advantages such as access to chemical synthesis ...

Wiley-V C H Verlag Gmbh2014

, , , ,

Photoswitchable ligands are powerful tools to control biological processes at high spatial and temporal resolution. Unfortunately, such ligands exist only for a limited number of proteins and their development by rational design is not trivial. We have dev ...

American Chemical Society2014

, , , ,

The disulfide bonds that form between two cysteine residues are important in defining and rigidifying the structures of proteins and peptides. In polypeptides containing multiple cysteine residues, disulfide isomerization can lead to multiple products with ...

Nature Publishing Group2014

Development of New Bicyclic Peptide Formats and their Application in Directed Evolution

Shiyu Chen

Cyclic peptides have a range of properties that make them attractive as therapeutics such as high binding affinity, good target selectivity and low toxicity. While most peptides in the clinic are derived from nature, various powerful in vitro evolution tec ...

EPFL2013

Bicyclic peptide ligands pulled out of cysteine-rich peptide libraries

Christian Heinis, Petr Leiman, Shiyu Chen, Jeremy Touati, Julia Morales Sanfrutos

Bicyclic peptide ligands were found to have good binding affinity and target specificity. However, the method applied to generate bicyclic ligands based on phage-peptide alkylation is technically complex and limits its application to specialized laboratori ...

2013

, ,

Chemical modification of phage libraries has allowed the in vitro evolution of ligands having properties not provided by natural polypeptides. The development of novel and more diverse chemical reactions on phage was hampered by the lack of analytical meth ...

2013

, , , , ,

Improving the binding affinity and/or stability of peptide ligands often requires testing of large numbers of variants to identify beneficial mutations. Herein we propose a type of mutation that promises a high success rate. In a bicyclic peptide inhibitor ...

2013

Pattern-Based Sensing of Peptides and Aminoglycosides with a Single Molecular Probe

Rosario Scopelliti, Kay Severin, Christian Heinis, Shiyu Chen, Bo Ram Lee

A coumarin-based molecular probe can be used for the sensing of peptides and aminoglycoside antibiotics. The probe reacts with the primary amine group(s) of the analytes to give a mixture of covalent adducts with distinct colors. Each analyte gives rise to ...

American Chemical Society2013

Bicyclic Peptide inhibitor reveals large contact interface with a protease target

Christian Heinis, Alessandro Angelini, Shiyu Chen, Jeremy Touati

From a large combinatorial library of chemically constrained bicyclic peptides we isolated a selective and potent (K(i) = 53 nM) inhibitor of human urokinase-type plasminogen activator (uPA) and crystallized the complex. This revealed an extended structure ...

2012