Publications de Sylvia Ho | EPFL Graph Search

Dynamics of CLIMP-63 S-acylation control ER morphology

Vassily Hatzimanikatis, Françoise Gisou van der Goot Grunberg, Graham Knott, Kathryn Hess Bellwald, Laurence Gouzi Abrami, Béatrice Kunz, Luciano Andres Abriata, Sylvia Ho, Catherine Maclachlan, Patrick Alain Sandoz

A key player in the formation of endoplasmic reticulum sheets is CLIMP-63, but mechanistic details remained elusive. Here authors combined cellular experiments and mathematical modelling to show that S-acylation of CLIMP-63 regulates its function by mediat ...

Nature Portfolio2023

CERT1 mutations perturb human development by disrupting sphingolipid homeostasis

Bruno Lemaitre, Paolo De Los Rios, Giovanni D'Angelo, Evelyne Ruchti, Sylvia Ho, Samuel Rommelaere, Charlotte Julie Caroline Gehin, Laura Capolupo, Greta Limoni, Huixin Guo

Neural differentiation, synaptic transmission, and action potential propagation depend on membrane sphingolipids, whose metabolism is tightly regulated. Mutations in the ceramide transporter CERT (CERT1), which is involved in sphingolipid biosynthesis, are ...

American Society for Clinical Investigation2023

Sphingolipids control dermal fibroblast heterogeneity

Giovanni D'Angelo, Gioele La Manno, Olivier Burri, Romain Guiet, Sylvia Ho, Alex Russell Lederer, Laura Capolupo, Irina Khven, Galina Glousker

Human cells produce thousands of lipids that change during cell differentiation and can vary across individual cells of the same type. However, we are only starting to characterize the function of these cell-to-cell differences in lipid composition. Here, ...

American Association for the Advancement of Science2022

Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chains

Françoise Gisou van der Goot Grunberg, Matteo Dal Peraro, Laurence Gouzi Abrami, Florence Pojer, Maria Josefina Marcaida Lopez, Sylvia Ho, Francisco De Magalhães Sarmento R De Mesquita, Muhammad Umair Anwar, Patrick Alain Sandoz, Martina Audagnotto

Acyl protein thioesterase APT2 interacts with membranes via its charged beta-tongue, becomes palmitoylated by ZDHHC3/7 and deforms the bilayer to extract substrate acyl chains. APT2 deacylation leads to its membrane release and degradation. ...

NATURE RESEARCH2021

Damage of eukaryotic cells by the pore-forming toxin sticholysin II: Consequences of the potassium efflux

Françoise Gisou van der Goot Grunberg, Sylvia Ho

Pore-forming toxins (PFTs) form holes in membranes causing one of the most catastrophic damages to a target cell. Target organisms have evolved a regulated response against PFTs damage including cell membrane repair. This ability of cells strongly depends ...

Elsevier Science Bv2017

New Insight into the Catalytic and Inhibition Mechanism of the Human Acyl Protein Thioesterase

Françoise Gisou van der Goot Grunberg, Martina Audagnotto, Matteo Dal Peraro, Sylvia Ho, Patrick Alain Sandoz, Nicole Andenmatten

Cell Press2016

Pore-forming toxins induce multiple cellular responses promoting survival

Françoise Gisou van der Goot Grunberg, Mirko Bischofberger, Rodrigo Manuel Gonzalez, Sylvia Ho

Pore-forming toxins (PFTs) are secreted proteins that contribute to the virulence of a great variety of bacterial pathogens. They inflict one of the more disastrous damages a target cell can be exposed to: disruption of plasma membrane integrity. Since thi ...

Wiley-Blackwell2011

Dual Chaperone Role of the C-Terminal Propeptide in Folding and Oligomerization of the Pore-Forming Toxin Aerolysin

Françoise Gisou van der Goot Grunberg, Matteo Dal Peraro, Mircea Ioan Iacovache, Sylvia Ho, Matteo Thomas Degiacomi

Throughout evolution, one of the most ancient forms of aggression between cells or organisms has been the production of proteins or peptides affecting the permeability of the target cell membrane. This class of virulence factors includes the largest family ...

2011