Publications de Charlène Mireille Raymonde Raclot

Cancer cells subvert the primate-specific KRAB zinc finger protein ZNF93 to control APOBEC3B

Didier Trono, Priscilla Turelli, Sandra Eloise Kjeldsen, Evaristo Jose Planet Letschert, Julien Léonard Duc, Filipe Amândio Brandão Sanches Vong Martins, Charlène Mireille Raymonde Raclot, Cyril David Son-Tuyên Pulver, Olga Marie Louise Rosspopoff, Romain Forey

ZNF93 is a primate-restricted Krüppel-associated box zinc finger protein responsible for repressing 20- to 12-My-old L1 transposable elements. Here, we reveal that ZNF93 also regulates the key cancer driver APOBEC3B—a mutagenic enzyme linked to tumorigenes ...

Proceedings of the National Academy of Sciences2025

Evolutionarily recent transcription factors partake in human cell cycle regulation

Didier Trono, Sandra Eloise Kjeldsen, Evaristo Jose Planet Letschert, Filipe Amândio Brandão Sanches Vong Martins, Charlène Mireille Raymonde Raclot, Alex Russell Lederer, Cyril David Son-Tuyên Pulver, Olga Marie Louise Rosspopoff, Romain Forey, Alexandre Coudray

The cell cycle is a fundamental process in eukaryotic biology and is accordingly controlled by a highly conserved core signaling cascade. However, whether recently evolved proteins also influence this process is unclear. Here, we systematically map the inf ...

2025

Broadly potent anti-SARS-CoV-2 antibody shares 93% of epitope with ACE2 and provides full protection in monkeys

Didier Trono, Henning Paul-Julius Stahlberg, Priscilla Turelli, Florence Pojer, Charlène Mireille Raymonde Raclot, Kelvin Ka Ching Lau, Yoan Désiré Duhoo, Jérémy Campos, Myriam Isabelle Lamrayah, Vanessa Genet

Due to the rapid evolution of SARS-CoV-2 to variants with reduced sensitivity to vaccine-induced humoral immunity and the near complete loss of protective efficacy of licensed therapeutic monoclonal antibodies, we isolated a potent, broad-spectrum neutrali ...

2023

De novo design of protein interactions with learned surface fingerprints

Didier Trono, Henning Paul-Julius Stahlberg, Beat Fierz, Priscilla Turelli, Bruno Emanuel Ferreira De Sousa Correia, Elisa Oricchio, Sandrine Madeleine Suzanne Georgeon, Dongchun Ni, Andreas Scheck, Michael Bronstein

Physical interactions between proteins are essential for most biological processes governing life. However, the molecular determinants of such interactions have been challenging to understand, even as genomic, proteomic and structural data increase. This k ...

2023

Patient-derived monoclonal antibody neutralizes SARS-CoV-2 Omicron variants and confers full protection in monkeys

Didier Trono, Henning Paul-Julius Stahlberg, Priscilla Turelli, Florence Pojer, Dongchun Ni, Charlène Mireille Raymonde Raclot, Kelvin Ka Ching Lau

The SARS-CoV-2 Omicron variant has very high levels of transmission, is resistant to neutralization by authorized therapeutic human monoclonal antibodies (mAb) and is less sensitive to vaccine-mediated immunity. To provide additional therapies against Omic ...

2022

S-acylation controls SARS-CoV-2 membrane lipid organization and enhances infectivity

Didier Trono, Françoise Gisou van der Goot Grunberg, Matteo Dal Peraro, Laurence Gouzi Abrami, Luciano Andres Abriata, Priscilla Turelli, Giovanni D'Angelo, Béatrice Kunz, Charlène Mireille Raymonde Raclot, Francisco De Magalhães Sarmento R De Mesquita

SARS-CoV-2 virions are surrounded by a lipid bilayer that contains membrane proteins such as spike, responsible for target-cell binding and virus fusion. We found that during SARS-CoV-2 infection, spike becomes lipid modified, through the sequential action ...

Cell Press - Elsevier2021

A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

Didier Trono, Priscilla Turelli, Florence Pojer, Charlène Mireille Raymonde Raclot, Valeria Cagno, Jérémy Campos, Céline Pellaton

The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies in the serum of an individual indicates previous infection or vaccination. However, it provides limited insight into the protective nature of this immune resp ...

AMER ASSOC ADVANCEMENT SCIENCE2021

A highly potent antibody effective against SARS-CoV-2 variants of concern

Didier Trono, Priscilla Turelli, Florence Pojer, Davide Demurtas, Charlène Mireille Raymonde Raclot, Kelvin Ka Ching Lau, Jérémy Campos

Control of the ongoing SARS-CoV-2 pandemic is endangered by the emergence of viral variants with increased transmission efficiency, resistance to marketed therapeutic antibodies, and reduced sensitivity to vaccine-induced immunity. Here, we screen B cells ...

2021

Primate-restricted KRAB zinc finger proteins and target retrotransposons control gene expression in human neurons

Didier Trono, Bart Deplancke, Priscilla Turelli, Christian Axel Wandall Thorball, Julien Léonard Duc, Charlène Mireille Raymonde Raclot, Alexandre Coudray, Julien Paul André Pontis, Delphine Yvette L Grun, Christopher James Playfoot

In the first days of embryogenesis, transposable element-embedded regulatory sequences (TEeRS) are silenced by Kruppel-associated box (KRAB) zinc finger proteins (KZFPs). Many TEeRS are subsequently co-opted in transcription networks, but how KZFPs influen ...

2020

The KRAB-ZFP/KAP1 System Contributes to the Early Embryonic Establishment of Site-Specific DNA Methylation Patterns Maintained during Development

Didier Trono, Priscilla Turelli, Sandra Eloise Kjeldsen, Adamantia Kapopoulou, Karolina Bojkowska, Charlène Mireille Raymonde Raclot

De novo DNA methylation is an essential aspect of the epigenetic reprogramming that takes place during early development, yet factors responsible for its instatement at particular genomic loci are poorly defined. Here, we demonstrate that the KRAB-ZFP-medi ...

Elsevier2012