Publications de Mukul Girotra | EPFL Graph Search

In Vivo Pre-Instructed HSCs Robustly Execute Asymmetric Cell Divisions In Vitro

Matthias Lütolf, Aline Roch, Mukul Girotra, Vincent Trachsel

Hematopoietic stem cells (HSCs) are responsible for life-long production of all mature blood cells. Under homeostasis, HSCs in their native bone marrow niches are believed to undergo asymmetric cell divisions (ACDs), with one daughter cell maintaining HSC ...

2020

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Olaia Maria Naveiras Torres-Quiroga, Mukul Girotra, George Coukos, Nicola Vannini, Alexandre Harari

A fine balance of quiescence, self-renewal, and differentiation is key to preserve the hematopoietic stem cell (HSC) pool and maintain lifelong production of all mature blood cells. In recent years cellular metabolism has emerged as a crucial regulator of ...

2019

The NAD-Booster Nicotinamide Riboside Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance

Johan Auwerx, Matthias Lütolf, Bart Deplancke, Carlos Canto Alvarez, Olaia Maria Naveiras Torres-Quiroga, George Coukos, Aurélien Laurent Jean-Charles Oggier, Dongryeol Ryu, Eija Riitta Pirinen, Loïc Tauzin

It has been recently shown that increased oxidative phosphorylation, as reflected by increased mitochondrial activity, together with impairment of the mitochondrial stress response, can severely compromise hematopoietic stem cell (HSC) regeneration. Here w ...

CELL PRESS2019

The NAD plus Salvage Pathway Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance and Asymmetric Division

Matthias Lütolf, Bart Deplancke, Carlos Canto Alvarez, Olaia Maria Naveiras Torres-Quiroga, Dongryeol Ryu, George Coukos, Aurélien Laurent Jean-Charles Oggier, Eija Riitta Pirinen, Vasco Ledebur de Antas de Campos, Loïc Tauzin

AMER SOC HEMATOLOGY2018

Methods & compounds useful in hematopoietic stem cell medicine

Matthias Lütolf, Johan Auwerx, Olaia Maria Naveiras Torres-Quiroga, Mukul Girotra, Nicola Vannini

The present invention relates to a specific marker for stem cells having long-term multi-lineage blood reconstitution capability and uses thereof for the preparation of cell composition in view of hematopoietic stem cell medicine and in particular regenera ...

2016

Specification of haematopoietic stem cell fate via modulation of mitochondrial activity

Johan Auwerx, Matthias Lütolf, Aline Roch, Olaia Maria Naveiras Torres-Quiroga, Mukul Girotra, Nicola Vannini, Vasco Ledebur de Antas de Campos, Gennady Nikitin, Sonja Giger

Haematopoietic stem cells (HSCs) differ from their committed progeny by relying primarily on anaerobic glycolysis rather than mitochondrial oxidative phosphorylation for energy production. However, whether this change in the metabolic program is the cause ...

Nature Publishing Group2016

Deciphering Metabolic Regulation of Hematopoietic Stem Cell Fate

Mukul Girotra

Hematopoietic stem cells (HSCs) are responsible for life-long production of all mature blood cells. This unique characteristic makes them an ideal candidate for cell-based therapies to treat various hematological malignancies. Their extensive use in the cl ...

EPFL2015

Mitochondrial Activity Determines Hematopoietic Stem Cell Fate Decisions

Matthias Lütolf, Johan Auwerx, Aline Roch, Mukul Girotra, Olaia Maria Naveiras Torres-Quiroga, Nicola Vannini, Vasco Ledebur de Antas de Campos

Amer Soc Hematology2014

Automated analysis of single stem cells in microfluidic traps

Matthias Lütolf, Alessandra Griffa, Arne Seitz, Olivier Burri, Stefan Kobel, Mukul Girotra

We report a reliable strategy to perform automated image cytometry of single (non-adherent) stem cells captured in microfluidic traps. The method rapidly segments images of an entire microfluidic chip based on the detection of horizontal edges of microflui ...

2012

In Vivo Modulation of Mitochondrial Activity Determines HSC Engraftment and Post-Transplant Survival in Mice

Matthias Lütolf, Johan Auwerx, Aline Roch, Mukul Girotra, Olaia Maria Naveiras Torres-Quiroga, Nicola Vannini, Richardus Houtkooper, Eija Riitta Pirinen, Vasco Ledebur de Antas de Campos

Amer Soc Hematology2012