Publications de Sergey Nazarov

Author Correction: Fully co-factor-free ClearTau platform produces seeding-competent Tau fibrils for reconstructing pathological Tau aggregates

Henning Paul-Julius Stahlberg, Hilal Lashuel, Sergey Nazarov, Meltem Tatli, Galina Limorenko, Rajasekhar Kolla

Nature Research2024

Cryo-EM structures and binding of mouse and human ACE2 to SARS-CoV-2 variants of concern indicate that mutations enabling immune escape could expand host range

Didier Trono, Henning Paul-Julius Stahlberg, Priscilla Turelli, Florence Pojer, Dongchun Ni, Sergey Nazarov, Kelvin Ka Ching Lau, Alexander Myasnikov, Emiko Uchikawa

nvestigation of potential hosts of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is crucial to understanding future risks of spillover and spillback. SARS-CoV-2 has been reported to be transmitted from humans to various animals after req ...

2023

Seeding the aggregation of TDP-43 requires post-fibrillization proteolytic cleavage

Hilal Lashuel, Sergey Nazarov, Urszula Beata Cendrowska, Niran Maharjan

Reconstitution of TDP-43 filaments that exhibit sequence and morphological features similar to those found in the brain helps to uncover a new mechanism for the formation and propagation of pathology in amyotrophic lateral sclerosis and other neurodegenera ...

2023

Structural insights into the regulation of Cas7-11 by TPR-CHAT

Henning Paul-Julius Stahlberg, Dongchun Ni, Sergey Nazarov, Alexander Myasnikov, Babatunde Edukpe Ekundayo

The CRISPR-guided caspase (Craspase) complex is an assembly of the target-specific RNA nuclease known as Cas7-11 bound to CRISPR RNA (crRNA) and an ancillary protein known as TPR-CHAT (tetratricopeptide repeats (TPR) fused with a CHAT domain). The Craspase ...

2023

Structural Basis of Huntingtin Fibril Polymorphism Revealed by Cryogenic Electron Microscopy of Exon 1 HTT Fibrils

Hilal Lashuel, Sergey Nazarov, Anass Chiki, Driss Boudeffa

The lack of detailed insight into the structure of aggregates formed by the huntingtin protein (HTT) has hampered the efforts to develop therapeutics and diagnostics targeting pathology formation in the brain of patients with Huntington's disease. To addre ...

2022

Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

Pierre Gönczy, Davide Demurtas, Georgios Hatzopoulos, Sergey Nazarov, Alexandra Bezler, Paul Guichard, Veronika Nemcíková Villímová

Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers ofSAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellateTrichonymphaspp. ...

2020

Structure and transformation of bacteriophage A511 baseplate and tail upon infection of Listeria cells

Sergey Nazarov, Petr Leiman, Ricardo Cesar Guerrero Ferreira

Contractile injection systems (bacteriophage tails, type VI secretions system, R-type pyocins, etc.) utilize a rigid tube/contractile sheath assembly for breaching the envelope of bacterial and eukaryotic cells. Among contractile injection systems, bacteri ...

WILEY2019

Structure and Function of the Branched Receptor-Binding Complex of Bacteriophage CBA120

Sergey Nazarov, Petr Leiman, Sergii Buth, Michel Plattner, Nikolai Prokhorov

Bacteriophages recognize their host cells with the help of tail fiber and tailspike proteins that bind, cleave, or modify certain structures on the cell surface. The spectrum of ligands to which the tail fibers and tailspikes can bind is the primary determ ...

Academic Press Ltd- Elsevier Science Ltd2019

Diverse roles of TssA-like proteins in the assembly of bacterial type VI secretion systems

Henning Paul-Julius Stahlberg, Sergey Nazarov

Protein translocation by the bacterial type VI secretion system (T6SS) is driven by a rapid contraction of a sheath assembled around a tube with associated effectors. Here, we show that TssA-like or TagA-like proteins with a conserved N-terminal domain and ...

2019

Genetic, structural and phenotypic properties of MS2 coliphage with resistance to ClO2 disinfection

Tamar Kohn, Anna Carratala Ripolles, Virginie Bachmann, Sergey Nazarov, Petr Leiman, Qingxia Zhong, Ricardo Cesar Guerrero Ferreira, Laura Piccinini

Common water disinfectants like chlorine have been reported to select for resistant viruses, yet little attention has been devoted to characterizing disinfection resistance. Here, we investigated the resistance of MS2 coliphage to inactivation by chlorine ...

Amer Chemical Soc2016