Publications de Roberto Buratto

Ligand-Protein Affinity Studies Using Long-Lived States of Fluorine-19 Nuclei

Geoffrey Bodenhausen, Daniele Mammoli, Roberto Buratto, Estel Canet Martinez

The lifetimes T-LLS of long-lived states or T-LLC of long-lived coherences can be used for the accurate determination of dissociation constants of weak protein ligand complexes. The remarkable contrast between signals derived from LLS or LLC in free and bo ...

American Chemical Society2016

Collisional cross-section of water molecules in vapour studied by means of H-1 relaxation in NMR

Geoffrey Bodenhausen, Lothar Helm, Pascal Miéville, Daniele Mammoli, Roberto Buratto, Estel Canet Martinez

In gas phase, collisions that affect the rotational angular momentum lead to the return of the magnetization to its equilibrium (relaxation) in Nuclear Magnetic Resonance (NMR). To the best of our knowledge, the longitudinal relaxation rates R-1 = 1/T-1 of ...

Nature Research2016

Challenges in preparing, preserving and detecting para-water in bulk: overcoming proton exchange and other hurdles

Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Diego Carnevale, Nicola Salvi, Daniele Mammoli, Roberto Buratto, Estel Canet Martinez

Para-water is an analogue of para-hydrogen, where the two proton spins are in a quantum state that is antisymmetric under permutation, also known as singlet state. The populations of the nuclear spin states in para-water are believed to have long lifetimes ...

Royal Society of Chemistry2015

Exploring Ligand Affinities for Proteins by NMR of Long-Lived States

Roberto Buratto

The detection of molecules that can bind to active sites of protein targets and the measurement of their affinities is a promising application of NMR. Nowadays, the screening of drug candidates is routinely done by NMR in pharmaceutical industry. We have p ...

EPFL2015

Drug Screening Boosted by Hyperpolarized Long-Lived States in NMR

Geoffrey Bodenhausen, Sandrine Gerber, Aurélien Bornet, Jonas Milani, Sami Jannin, Basile Vuichoud, Solène Marcelle Françoise Marie Passemard, Nicola Salvi, Daniele Mammoli, Roberto Buratto

Transverse and longitudinal relaxation times (T11 and T1) have been widely exploited in NMR to probe the binding of ligands and putative drugs to target proteins. We have shown recently that long-lived states (LLS) can be more sensitive to ligand binding. ...

Wiley-Blackwell2014

Exploring Weak Ligand-Protein Interactions by Long-Lived NMR States: Improved Contrast in Fragment-Based Drug Screening

Geoffrey Bodenhausen, Daniele Mammoli, Roberto Buratto

Ligands that have an affinity for protein targets can be screened very effectively by exploiting favorable properties of long-lived states (LLS) in NMR spectroscopy. In this work, we describe the use of LLS for competitive binding experiments to measure ac ...

Wiley-Blackwell2014

Method for the nmr based determination of the affinity of drugs for a target protein

Geoffrey Bodenhausen, Aurélien Bornet, Nicola Salvi, Roberto Buratto

The document relates to a method for the determination of the binding properties of at least one first chemical compound to at least one second chemical compound between a free essentially dissociated state and a bound essentially associated state by using ...

2013

Boosting the Sensitivity of Ligand-Protein Screening by NMR of Long-Lived States

Geoffrey Bodenhausen, Christian Heinis, Aurélien Bornet, Inmaculada Rentero Rebollo, Alessandro Angelini, Simone Ulzega, Nicola Salvi, Roberto Buratto

A new NMR method for the study of ligand protein interactions exploits the unusual lifetimes of long-lived states (LLSs). The new method provides better contrast between bound and free ligands and requires a protein-ligand ratio ca. 25 times lower than for ...

2012

Dynamic Nuclear Polarization and Other Magnetic Ideas at EPFL

Geoffrey Bodenhausen, Aurélien Bornet, Pascal Miéville, Sami Jannin, Jonas Milani, Diego Carnevale, Simone Ulzega, Marc Anthony Caporini, Nicola Salvi, Daniele Mammoli

Although nuclear magnetic resonance (NMR) can provide a wealth of information, it often suffers from a lack of sensitivity. Dynamic Nuclear Polarization (DNP) provides a way to increase the polarization and hence the signal intensities in NMR spectra by tr ...

2012