Publications de Thomas Pengo | EPFL Graph Search

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

Michaël Unser, Daniel Sage, Thanh-An Michel Pham, Tomas Lukes, Seamus John Holden, Anna Archetti, Thomas Pengo, Ricardo Henriques, Silvia Colabrese, Anurag Agrawal

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule localization microscopy (SMLM), a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effo ...

2019

Quantitative evaluation of software packages for single-molecule localization microscopy

Michaël Unser, Daniel Sage, Suliana Manley, Hagay Kirshner, Thomas Pengo

The quality of super-resolution images obtained by single-molecule localization microscopy (SMLM) depends largely on the software used to detect and accurately localize point sources. In this work, we focus on the computational aspects of super-resolution ...

Nature Publishing Group2015

PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy

Suliana Manley, Seamus John Holden, Thomas Pengo

During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful re ...

Oxford University Press2015

High throughput 3D Palm Imaging Elucidates Mechanisms of Bacterial Cell Division

Suliana Manley, Seamus John Holden, Thomas Pengo

Cell Press2014

High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization

Suliana Manley, Karin Lederballe Meibom, Seamus John Holden, Thomas Pengo

We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscal ...

National Academy of Sciences2014

Quantitative super-resolution imaging reveals protein stoichiometry and nanoscale morphology of assembling HIV-Gag virions

Suliana Manley, Thomas Pengo, Julia Gunzenhäuser

The HIV structural protein Gag assembles to form spherical particles of radius ∼70 nm. During the assembly process, the number of Gag proteins increases over several orders of magnitude from a few at nucleation to thousands at completion. The challenge in ...

American Chemical Society (ACS)2012

A Psf-Based Approach To Biplane Calibration In 3D Super-Resolution Microscopy

Michaël Unser, Suliana Manley, Daniel Sage, Hagay Kirshner, Thomas Pengo

Super-resolution localization microscopy methods such as PALM and STORM have been shown to provide imaging with resolutions up to a few tens of nanometers while using relatively simple setups. Biplane PALM has extended the PALM technique to three-dimension ...

Ieee2012