Publications de Bohumil Maco | EPFL Graph Search

Stable isotope labeling and ultra-high-resolution NanoSIMS imaging reveal alpha-synuclein-induced changes in neuronal metabolism in vivo

Anders Meibom, Bernard Schneider, Graham Knott, Stéphane Laurent Escrig, Louise Helene Soegaard Jensen, Bohumil Maco, Sofia Carmen Suzanne Spataro

In Parkinson's disease, pathogenic factors such as the intraneuronal accumulation of the protein alpha-synuclein affect key metabolic processes. New approaches are required to understand how metabolic dysregulations cause degeneration of vulnerable subtype ...

2023

The effects of aging on neuropil structure in mouse somatosensory cortex-A 3D electron microscopy analysis of layer 1

Pascal Fua, Marco Cantoni, Graham Knott, Bohumil Maco, Anne Alison Jorstad, Marta Wawrzyniak

2018

Multiphoton microscopy in brain imaging

Graham Knott, Bohumil Maco, Ludovico Silvestri

Brain imaging is becoming an important field in the frame of the neurophotonics in correlations with other medical ones in neuroscience studying functional and morphological aspects. In this presentation an overview on multi photon imaging of the brain wil ...

Spie-Int Soc Optical Engineering2015

The Relationship between PSD-95 Clustering and Spine Stability In Vivo

Graham Knott, Bohumil Maco

The appearance and disappearance of dendritic spines, accompanied by synapse formation and elimination may underlie the experience-dependent reorganization of cortical circuits. The exact temporal relationship between spine and synapse formation in vivo re ...

Soc Neuroscience2014

Semiautomated correlative 3D electron microscopy of in vivo-imaged axons and dendrites

Marco Cantoni, Graham Knott, Bohumil Maco

This protocol describes how in vivo-imaged dendrites and axons in adult mouse brains can subsequently be prepared and imaged with focused ion beam scanning electron microscopy (FIBSESEM). The procedure starts after in vivo imaging with chemical fixation, f ...

Nature Publishing Group2014

Correlative In Vivo 2-Photon Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure

Pascal Fua, Graham Knott, Bohumil Maco, Anne Alison Jorstad

This protocol describes how dendrites and axons, imaged in vivo, can subsequently be analyzed in 3D using focused ion beam scanning electron microscopy (FIBSEM). The fluorescent structures are identified after chemical fixation and their position highlight ...

Elsevier Academic Press Inc2014

In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits

Graham Knott, Bohumil Maco

To what extent, how and when axons respond to injury in the highly interconnected grey matter is poorly understood. Here we use two-photon imaging and focused ion beam-scanning electron microscopy to explore, at synaptic resolution, the regrowth capacity o ...

Nature Research2013

Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons

Marco Cantoni, Graham Knott, Bohumil Maco

Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, pre ...

Public Library of Science2013

Altered Synaptic Dynamics during Normal Brain Aging

Graham Knott, Bohumil Maco

What is the neuroanatomical basis for the decline in brain function that occurs during normal aging? Previous postmortem studies have blamed it on a reduction in spine density, though results remain controversial and spine dynamics were not assessed. We us ...

Soc Neuroscience2013