Publications de Qiuliyang Yu | EPFL Graph Search

A biosensor for measuring NAD(+) levels at the point of care

Johan Auwerx, Kai Johnsson, Luc Patiny, Elena Katsyuba, Qiuliyang Yu, Lin Xue

The cellular level of nicotinamide adenine dinucleotide (NAD(+)), through its different functions, affects cellular metabolism and signalling(1-3). A decrease in the NAD(+) content has been associated with various pathologies and physiological aging(4,5), ...

NATURE PUBLISHING GROUP2019

Semisynthetic sensor proteins enable metabolic assays at the point of care

Kai Johnsson, Rudolf Griss, Qiuliyang Yu, Lin Xue, Julien Hiblot

Monitoring metabolites at the point of care could improve the diagnosis and management of numerous diseases. Yet for most metabolites, such assays are not available. We introduce semisynthetic, light-emitting sensor proteins for use in paper-based metaboli ...

American Association for the Advancement of Science2018

Sensors, methods and kits for detecting nadph based on resonance energy transfer

Kai Johnsson, Qiuliyang Yu

The invention relates to the detection of the cofactor reduced nicotinamide adenine dinucleotide phosphate (NADPH). Provided is a sensor molecule for the resonance energy transfer (RET)-based detection of NADPH, the sensor comprising a segment A connected ...

2018

Cofactor sensing proteins for metabolic assays at point-of-care

Qiuliyang Yu

Miniaturized blood analysis promises a substantial improvement for the process of healthcare. However, most current blood analysis methods were developed in the context of centralized laboratories and there is a lack of robust detection technologies suitab ...

EPFL2018

Bioluminescent Antibodies for Point-of-Care Diagnostics

Kai Johnsson, Rudolf Griss, Alberto Schena, Qiuliyang Yu, Lin Xue

We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no-wash quantification of analytes. Our approach is based on the genetic fusion of antibody fragments to NanoLuc luciferase and SNAP-tag, the lat ...

Wiley-VCH Verlag GmbH2017

Highly Modular Bioluminescent Sensors for Small Molecules and Proteins

Kai Johnsson, Rudolf Griss, Alberto Schena, Qiuliyang Yu

Obtaining patient-specific information through the quantification of small molecules and proteins in bodily fluids is essential for personalized therapies. Point-of-care (POC) diagnostic devices hold the promise of delivering such benefit to a wide range o ...

Elsevier Academic Press Inc2017

Luciferases with tunable emission wavelengths

Kai Johnsson, Luc Reymond, Rudolf Griss, Alberto Schena, Qiuliyang Yu, Olivier Vincent Sallin, Lin Xue, Julien Hiblot, Nicholas Clarke Hill, Marina Daniela Beatrice Sabbadini

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. These tunable luciferases are generated by inserting the small luciferase NanoLuc into the structures of the self-labeling proteins SNAP-tag or HaloT ...

Wiley-Blackwell2017

On-Chip Spyhole Mass Spectrometry for Droplet-Based Microfluidics

Hubert Girault, Natalia Gasilova, Liang Qiao, Qiuliyang Yu

For efficient coupling of droplet-based microfluidics with mass spectrometry (MS), a spyhole drilled on the top of a microchip is used to sample the passing droplets by electrostatic-spray ionization (ESTASI) MS. The technique involves placing an electrode ...

Wiley-VCH Verlag GmbH2014