Publications de Saba Rezakhani

Bioengineered Tubular Biliary Organoids

Matthias Lütolf, Mikhail Nikolaev, Bilge Sen Elçi, Saba Rezakhani

Liver organoids have emerged as promising in vitro models for toxicology, drug discovery, and disease modeling. However, conventional 3D epithelial organoid culture systems suffer from significant drawbacks, including limited culture duration, a nonphysiol ...

Wiley2024

Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice

Matthias Lütolf, Antonius Chrisnandy, Saba Rezakhani

Glandular epithelia, including mammary gland (MG) and prostate, are composed of luminal and basal cells. During embryonic development, glandular epithelia arise from multipotent stem cells (SCs) that are replaced after birth by unipotent basal and unipoten ...

Nature Research2024

Embryo-uterine interaction coordinates mouse embryogenesis during implantation

Matthias Lütolf, Mikhail Nikolaev, Saba Rezakhani

Embryo implantation into the uterus marks a key transition in mammalian development. In mice, implantation is mediated by the trophoblast and is accompanied by a morphological transition from the blastocyst to the egg cylinder. However, the roles of tropho ...

WILEY2023

Deterministic scRNA-seq captures variation in intestinal crypt and organoid composition

Matthias Lütolf, Bart Deplancke, Esther Amstad, Vincent Roland Julien Gardeux, Julie Marie Russeil, Rita Sarkis, Jörn Pezoldt, Johannes Julius Bues, Antonius Chrisnandy, Riccardo Dainese

DisCo is a deterministic droplet microfluidics tool for single-cell analysis on low cell input samples, which is demonstrated to profile individual intestinal organoids and in vivo-derived small tissues. ...

NATURE PORTFOLIO2022

Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis

Matthias Lütolf, Nicolas Antoine Vincent Broguiere, Antonius Chrisnandy, Saba Rezakhani, Delphine Lorraine Perle Blondel

Epithelial organoids are most efficiently grown from mouse-tumour-derived, reconstituted extracellular matrix hydrogels, whose poorly defined composition, batch-to-batch variability and immunogenicity limit clinical applications. Efforts to replace such il ...

NATURE PORTFOLIO2022

A functional genetic toolbox for human tissue-derived organoids

Matthias Lütolf, Saba Rezakhani

Human organoid systems recapitulate key features of organs offering platforms for modelling developmental biology and disease. Tissue-derived organoids have been widely used to study the impact of extrinsic niche factors on stem cells. However, they are ra ...

eLIFE SCIENCES PUBL LTD2021

Defined matrices for epithelial organoid culture and disease modelling

Saba Rezakhani

Advances in our understanding of the biology of stem cells and their niches have provided the necessary foundation to create in vitro complex self-organizing three-dimensional (3D) structures called organoids. While organoids represent a highly promising n ...

EPFL2021

Mechano-modulatory synthetic niches for liver organoid derivation

Kristina Schoonjans, Matthias Lütolf, Giovanni Sorrentino, Saba Rezakhani, Ece Yildiz

The recent demonstration that primary cells from the liver can be expanded in vitro as organoids holds enormous promise for regenerative medicine and disease modelling. The use of three-dimensional (3D) cultures based on ill-defined and potentially immunog ...

Nature Publishing Group2020

Low-Defect Thiol-Michael Addition Hydrogels as Matrigel Substitutes for Epithelial Organoid Derivation

Matthias Lütolf, Nikolche Gjorevski, Saba Rezakhani

Due to their selective crosslinking chemistry and well-defined mechano-biochemical characteristics, hydrogels crosslinked by thiol-Michael addition reactions have garnered immense interest for 3D cell culture applications. These hydrogels are typically for ...

2020

Three dimensional models of tissue fibrosis

Matthias Lütolf, Kristina Schoonjans, Giovanni Sorrentino, Saba Rezakhani, Ece Yildiz

The invention provides a method for obtaining a three-dimensional (3D) cellular model of tissue fibrosis, such as liver fibrosis, comprising culturing epithelial cells in a 3D hydrogel in conditions suitable for a three-dimensional cellular cluster formati ...

2020