Personne
Kristina Srzentic
Cette personne n’est plus à l’EPFL
Publications associées (9)
Inter-laboratory Study for Characterizing Monoclonal Antibodies by Top-Down and Middle-Down Mass Spectrometry
Yury Tsybin, Natalia Gasilova, Laure Menin, Luca Fornelli, Kristina Srzentic
The Consortium for Top-Down Proteomics (www.topdownproteomics.org) launched the present study to assess the current state of top-down mass spectrometry (TD MS) and middle-down mass spectrometry (MD MS) for characterizing monoclonal antibody (mAb) primary s ...
2020Multiplexed Middle-Down Mass Spectrometry as a Method for Revealing Light and Heavy Chain Connectivity in a Monoclonal Antibody
Yury Tsybin, Natalia Gasilova, Laure Menin, Anton Kozhinov, Konstantin Nagornov, Luca Fornelli, Kristina Srzentic, Daniel Ayoub
Pairing light and heavy chains in monoclonal antibodies (mAbs) using top-down (TD) or middle-down (MD) mass spectrometry (MS) may complement the sequence information on single chains provided by high-throughput genomic sequencing and bottom-up proteomics, ...
AMER CHEMICAL SOC2018Monitoring Membrane Lipidome Turnover by Metabolic N-15 Labeling and Shotgun Ultra-High-Resolution Orbitrap Fourier Transform Mass Spectrometry
Yury Tsybin, Konstantin Nagornov, Kristina Srzentic
Lipidomes undergo permanent extensive remodeling, but how the turnover rate differs between lipid classes and molecular species is poorly understood. We employed metabolic N-15 labeling and shotgun ultra-high-resolution mass spectrometry (sUHR) to quantify ...
Amer Chemical Soc2017Middle-down approaches for mass spectrometry-based protein identification and characterization
Mass spectrometry (MS) has emerged over the last two decades as the analytical technique of choice in systems-level protein studies, known as proteomics. Two are the MS-based approaches generally applied to proteomics: bottom-up (BU), which relies on the p ...
EPFL2016On-chip mesoporous functionalized magnetic microspheres for protein sequencing by extended bottom-up mass spectrometry
Hubert Girault, Yury Tsybin, Natalia Gasilova, Liang Qiao, Kristina Srzentic
Limited amount and extreme concentration variability of proteomic-related samples require efficient analyte preconcentration and purification prior to the mass spectrometry (MS)-based analysis. Preferably, these steps should be coupled on-line with chosen ...
American Chemical Society2016Advantages of Extended Bottom-Up Proteomics Using Sap9 for Analysis of Monoclonal Antibodies
Yury Tsybin, Luca Fornelli, Kristina Srzentic, Unige Anna Laskay, Daniel Ayoub
Despite the recent advances in structural analysis of monoclonal antibodies with bottom-up, middle-down, and top-down mass spectrometry (MS), further improvements in analysis accuracy, depth, and speed are needed. The remaining challenges include quantitat ...
Amer Chemical Soc2014Extended Bottom-Up Proteomics with Secreted Aspartic Protease Sap9
Yury Tsybin, Kristina Srzentic, Unige Anna Laskay
We investigate the benefits and experimental feasibility of approaches enabling the shift from short (1.7 kDa on average) peptides in bottom-up proteomics to about twice longer (similar to 3.2 kDa on average) peptides in the so-called extended bottom-up pr ...
Elsevier Science Bv2014Practical Considerations for Improving the Productivity of Mass Spectrometry-based Proteomics
Yury Tsybin, Anton Kozhinov, Luca Fornelli, Kristina Srzentic, Unige Anna Laskay, Oxana Upir
Mass spectrometry (MS) is currently the most sensitive and selective analytical technique for routine peptide and protein structure analysis. Top-down proteomics is based on tandem mass spectrometry (MS/MS) of intact proteins, where multiply charged precur ...
Schweizerische Chemische Gesellschaft2013Proteome Digestion Specificity Analysis for Rational Design of Extended Bottom-up and Middle-down Proteomics Experiments
Yury Tsybin, Kristina Srzentic, Unige Anna Laskay
Mass spectrometry (MS)-based bottom-up proteomics (BUP) is currently the method of choice for large-scale identification and characterization of proteins present in complex samples, such as cell lysates, body fluids, or tissues. Technically, BUP relies on ...
Amer Chemical Soc2013