Publications de Virginija Jovaisaite

Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA

Although all Type II restriction endonucleases catalyze phosphodiester bond hydrolysis within or close to their DNA target sites, they form different oligomeric assemblies ranging from monomers, dimers, tetramers to higher order oligomers to generate a dou ...

Oxford University Press (OUP)2017

Track C. Biomedical Instrumentation & Micro and Nano Systems

Johan Auwerx, Matteo Cornaglia, Martinus Gijs, Thomas Lehnert, Laurent Mouchiroud, Vincenzo Sorrentino, Alexis Pierre Henri Marette, Virginija Jovaisaite, Govindan Narayanan Krishnamani, Shreya Narasimhan

De Gruyter2016

Mitochondrial unfolded protein response, its molecular mechanism and physiological impact

Virginija Jovaisaite

Mitochondria are the main producers of ATP, the energy currency of the cell, and they perform a wide array of other fundamental functions. These organelles are essential not only for cellular metabolism, but also for organismal physiology and lifespan. A r ...

EPFL2016

Two Conserved Histone Demethylases Regulate Mitochondrial Stress-Induced Longevity

Johan Auwerx, Laurent Mouchiroud, Evan Graehl Williams, Virginija Jovaisaite

Across eukaryotic species, mild mitochondrial stress can have beneficial effects on the lifespan of organisms. Mitochondrial dysfunction activates an unfolded protein response (UPR(mt)), a stress signaling mechanism designed to ensure mitochondrial homeost ...

Cell Press2016

The mitochondrial unfolded protein response - synchronizing genomes

Johan Auwerx, Virginija Jovaisaite

Maintenance of the mitochondrial proteome is performed primarily by chaperones, which fold and assemble proteins, and by proteases, which degrade excess damaged proteins. Upon various types of mitochondrial stress, triggered genetically or pharmacologicall ...

Current Biology Ltd2015

An automated microfluidic platform for C. elegans embryo arraying, phenotyping, and long-term live imaging

Johan Auwerx, Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Laurent Mouchiroud, Alexis Pierre Henri Marette, Virginija Jovaisaite, Shreya Narasimhan

Studies of the real-time dynamics of embryonic development require a gentle embryo handling method, the possibility of long-term live imaging during the complete embryogenesis, as well as of parallelization providing a population's statistics, while keepin ...

Nature Publishing Group2015

Tetracyclines Disturb Mitochondrial Function across Eukaryotic Models: A Call for Caution in Biomedical Research

Johan Auwerx, Bart Deplancke, Richardus Houtkooper, Laurent Mouchiroud, Dongryeol Ryu, Xu Wang, Evan Graehl Williams, Adrienne Joëlle Laurence Mottis, Virginija Jovaisaite, Norman Lionel Moullan

In recent years, tetracyclines, such as doxycycline, have become broadly used to control gene expression by virtue of the Tet-on/Tet-off systems. However, the wide range of direct effects of tetracycline use has not been fully appreciated. We show here tha ...

Elsevier2015

The mitochondrial unfolded protein response in mammalian physiology

Johan Auwerx, Adrienne Joëlle Laurence Mottis, Virginija Jovaisaite

Mitochondria, the main site of cellular energy harvesting, are derived from proteobacteria that evolved within our cells in endosymbiosis. Mitochondria retained vestiges of their proteobacterial genome, the circular mitochondrial DNA, which encodes 13 subu ...

Springer Verlag2014

The mitochondrial unfolded protein response, a conserved stress response pathway with implications in health and disease

Johan Auwerx, Laurent Mouchiroud, Virginija Jovaisaite

The ability to respond to various intracellular and/or extracellular stresses allows the organism to adapt to changing environmental conditions and drives evolution. It is now well accepted that a progressive decline of the efficiency of stress response pa ...

Company of Biologists2014

Multilayered genetic and omics dissection of mitochondrial activity in a mouse reference population

Johan Auwerx, Evan Graehl Williams, Adrienne Joëlle Laurence Mottis, Virginija Jovaisaite, Ruedi Aebersold, Sébastien Dubuis

The manner by which genotype and environment affect complex phenotypes is one of the fundamental questions in biology. In this study, we quantified the transcriptome--a subset of the metabolome--and, using targeted proteomics, quantified a subset of the li ...

Cell Press2014