Publications de Andrea Callegari

Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation

Suliana Manley, Beat Fierz, David Michael Suter, Christian Sieben, Aleksandr Benke, Andrea Callegari

Transcription factors (TFs) regulate gene expression in both prokaryotes and eukaryotes by recognizing and binding to specific DNA promoter sequences. In higher eukaryotes, it remains unclear how the duration of TF binding to DNA relates to downstream tran ...

2019

A role for mitotic bookmarking of SOX2 in pluripotency and differentiation

Suliana Manley, Aleksandr Benke, David Michael Suter, Marion Leleu, Andrea Callegari, Cédric Deluz, Torgil Elias Friman, Mahé Cécile Raccaud, Daniel Strebinger

Mitotic bookmarking transcription factors remain bound to chromosomes during mitosis and were proposed to regulate phenotypic maintenance of stem and progenitor cells at the mitosis-to-G1 (M-G1) transition. However, mitotic bookmarking remains largely unex ...

Cold Spring Harbor Laboratory2016

Eukaryotic transcription factor binding kinetics : a single-molecule and functional study

Andrea Callegari

Transcription represents a key regulatory step in gene expression and transcription factors (TFs) are the key molecular players of this process. TFs inhibit or promote the assembly of the transcriptional machinery by binding DNA and several co-factors. Whe ...

EPFL2016

Dipole Moment of Water in Highly Vibrationally Excited States: Analysis of Photofragment Quantum-Beat Spectroscopy Measurements Using a Local-Mode Hamiltonian

Patrice Theulé, Andrea Callegari

We present here the analysis of experimental Stark effect measurements made using photofragment quantum beat spectroscopy on the 14,0->, 15,0->, 18,0+> and 14,0-> 12 > vibrational states of H2O [Callegari, A.; et al. Science 2002, 297, 993.]. To link the m ...

2009

Relaxation processes of point defects in vitreous silica from femtosecond to nanoseconds

Majed Chergui, Frank van Mourik, Andrea Cannizzo, Wojciech Gawelda, Erwin Portuondo Campa, Andrea Callegari

The authors studied ultrafast relaxation of localized excited states at Ge-related O deficient centers in SiO2 using femtosecond transient-absorption spectroscopy. The relaxation dynamics exhibits a biexponential decay, which the authors ascribe to the dep ...

2008

A simple and accurate method for calibrating the oscillation amplitude of tuning-fork based AFM sensors

Majed Chergui, Jinquan Liu, Andrea Callegari

We have developed a simple and accurate method for calibrating the amplitude of vibration of quartz tuning fork sensors commonly used in at. force- and near field optical-microscopy. Unlike interferometric methods, which require a complex optical setup, th ...

2008

Time-Resolved Photodynamics of Triangular-Shaped Silver Nanoplates

Majed Chergui, Frank van Mourik, Camilla Bonati, Andrea Callegari, Luigi Bonacina

The authors measured the ultrafast response of triangular silver nanoparticles upon femtosecond excitation of their plasmon resonance. After a fast electron relaxation, the signature of a bimodal mech. vibration of the particle is apparent as a spectral mo ...

2006

Q-factor optimization of a tuning-fork/fiber sensor for shear-force detection

Majed Chergui, Jinquan Liu, Andrea Callegari

We present the results of an exptl. and theor. study on the optimum design of shear-force sensors, used in scanning probe microscopes. We have optimized a configuration consisting of a tuning-fork/fiber-tip assembly, achieving quality factors (Q) exceeding ...

AIP American Institute of Physics2005