Publications de Jonathan Bastien Cottet

Planar hydrodynamic traps and buried channels for bead and cell trapping and releasing

Philippe Renaud, Arnaud Bertsch, Daniel Migliozzi, Clémentine Sophie Sarah Lipp, Jonathan Bastien Cottet

We present a novel concept for the controlled trapping and releasing of beads and cells in a PDMS microfluidic channel without obstacles present around the particle or in the channel. The trapping principle relies on a two-level microfluidic configuration: ...

ROYAL SOC CHEMISTRY2021

Positional dependence of particles and cells in microfluidic electrical impedance flow cytometry: origin, challenges and opportunities

Philippe Renaud, Jonathan Bastien Cottet

Microfluidic electrical impedance flow cytometry is now a well-known and established method for single-cell analysis. Given the richness of the information provided by impedance measurements, this non-invasive and label-free approach can be used in a wide ...

2020

Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes

Philippe Renaud, Harald Van Lintel, Jonathan Bastien Cottet, Soufian Lasli

We present a microfluidic platform allowing dielectrophoresis-assisted formation of cell aggregates of controlled size and composition under flow conditions. When specific ex- perimental conditions are met, negative dielectrophoresis allows efficient conce ...

2019

How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study

Philippe Renaud, Jonathan Bastien Cottet, Harald Van Lintel

This paper describes a comprehensive analysis of the geometrical parameters in uencing the sensitivity of a coplanar elec- trode layout for electrical impedance ow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3 ...

2019

MyDEP: A New Computational Tool for Dielectric Modeling of Particles and Cells

Philippe Renaud, Jonathan Bastien Cottet

Dielectrophoresis (DEP) and electrorotation (ROT) are two electrokinetic phenomena exploiting nonuniform electric fields to exert a force or torque on biological particles suspended in liquid media. They are widely used in lab-on-chip devices for the manip ...

2019

Impedance-based real-time position sensor for lab-on-a-chip devices

Philippe Renaud, Harald Van Lintel, Jonathan Bastien Cottet

This paper presents the theoretical and experimental development of an integrated position sensor for lab-on-a-chip devices. The interest for single cell analysis is growing. However, this requires monitoring and controlling cell displacements in real time ...

2018

MyDEP: A New Computational Tool For Dielectric Modeling Of Particles And Cells

Philippe Renaud, Jonathan Bastien Cottet

Before performing experiments in the lab with dielectrophoresis (DEP), it is useful to predict the particle and cell responses to the electric field. This requires knowledge of particles or cells properties, which can be obtained from the literature, and i ...

2018

A reproducible method for μm precision alignment of PDMS microchannels with on-chip electrodes using a mask aligner

Philippe Renaud, Jonathan Bastien Cottet

This paper describes a reproducible method for μm precision alignment of polydimethylsiloxane (PDMS) microchannels with coplanar electrodes using a conventional mask aligner for lab-on-a-chip applications. It is based on the use of a silicon mold in combin ...

American Institute of Physics2017

Creation of cell aggregates by dielectrophoresis for the in vitro study of electrochemotherapy

Philippe Renaud, Jonathan Bastien Cottet

2016

Dielectrophoretic formation of cell aggregates mimicking tumors for in vitro study of electrochemotherapy

Philippe Renaud, Jonathan Bastien Cottet

2016