Publications de Kyle Michael Douglass

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Khalid Ashraf Mohie Ibrahim, Davide Gambarotto, Paul Guichard, Virginie Hamel, Niccolo Banterle, Dora Mahecic, Denis Fortun

Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...

2020

Koehler integrator device and application thereof in a multi-focal confocal microscope

Suliana Manley, Kyle Michael Douglass, Dora Mahecic

A Koehler integrator device (10) comprises a collimating lens (11) being arranged for collimating a light field created by an incoherent or partially coherent light source, a pair of planar first and second micro-lens arrays (12, 13) being arranged for rel ...

2020

Multicolor Single-Particle Reconstruction of Protein Complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

CELL PRESS2019

EZH2 oncogenic mutations drive epigenetic, transcriptional, and structural changes within chromatin domains

Suliana Manley, Elisa Oricchio, Kyle Michael Douglass, Natalya Katanayeva, Daniele Tavernari, Elena Battistello, Stephanie Jocelyne Sungalee, Maria Christine Donaldson, Timo Henry René Rey, Giovanni Ciriello

Chromatin is organized into topologically associating domains (TADs) enriched in distinct histone marks. In cancer, gain-of-function mutations in the gene encoding the enhancer of zeste homolog 2 protein (EZH2) lead to a genome-wide increase in histone-3 L ...

2019

Autonomous illumination control for localization microscopy

Suliana Manley, Kyle Michael Douglass, Baptiste Romain Samuel Ottino, Marcel Stefko

Super-resolution fluorescence microscopy improves spatial resolution, but this comes at a loss of image throughput and presents unique challenges in identifying optimal acquisition parameters. Microscope automation routines can offset these drawbacks, but ...

OPTICAL SOC AMER2018

Super-resolution microscopy to decipher multi-molecular assemblies

Suliana Manley, Kyle Michael Douglass, Paul Guichard, Christian Sieben

2018

Multicolor single-particle reconstruction of protein complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...

2018

The telomeric DNA damage response occurs in the absence of chromatin decompaction

Joachim Lingner, Suliana Manley, Verena Pfeiffer, Aleksandra Vancevska, Kyle Michael Douglass

Telomeres are specialized nucleoprotein structures that protect chromosome ends from DNA damage response (DDR) and DNA rearrangements. The telomeric shelterin protein TRF2 suppresses the DDR, and this function has been attributed to its abilities to trigge ...

Cold Spring Harbor Laboratory2017

Super-resolution imaging of multiple cells by optimized flat-field epi-illumination

Suliana Manley, Kyle Michael Douglass, Christian Sieben, Anna Archetti, Ambroise Justin Lambert

Biological processes are inherently multi-scale, and supramolecular complexes at the nanoscale determine changes at the cellular scale and beyond. Single-molecule localizationmicroscopy (SMLM)(1-3) techniques have been established as important tools for st ...

Nature Publishing Group2016

Nanoscale Spatial Organization of Chromatin in its Cellular Context, from Telomeres to Hox

Denis Duboule, Joachim Lingner, Suliana Manley, Kyle Michael Douglass, Aleksandr Benke, Verena Pfeiffer, Pierre Raymond Fabre, Aleksandra Vancevska

Cell Press2016