Ece Ozelci

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Actuated 3D microgels for single cell mechanobiology

Ece Ozelci, Berna Ayse Özkale Edelmann, Mahmut Selman Sakar, Christina Myra Tringides

We present a new cell culture technology for large-scale mechanobiology studies capable of generating and applying optically controlled uniform compression on single cells in 3D. Mesenchymal stem cells (MSCs) are individually encapsulated inside an optically triggered nanoactuator-alginate hybrid biomaterial using microfluidics, and the encapsulating network isotropically compresses the cell upon activation by light. The favorable biomolecular properties of alginate allow cell culture in vitro up to a week. The mechanically active microgels are capable of generating up to 15% compressive strain and forces reaching 400 nN. As a proof of concept, we demonstrate the use of the mechanically active cell culture system in mechanobiology by subjecting singly encapsulated MSCs to optically generated isotropic compression and monitoring changes in intracellular calcium intensity.

ROYAL SOC CHEMISTRY2022

Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation

Erik Mailand, Andrew Charles Oates, Ece Ozelci, Matthias Rüegg, Mahmut Selman Sakar

Classic microsurgical techniques, such as those used in the early 1900s by Mangold and Spemann, have been instrumental in advancing our understanding of embryonic development. However, these techniques are highly specialized, leading to issues of inter-operator variability. Here we introduce a user-friendly robotic microsurgery platform that allows precise mechanical manipulation of soft tissues in zebrafish embryos. Using our platform, we reproducibly targeted precise regions of tail explants, and quantified the response in real-time by following notochord and presomitic mesoderm (PSM) morphogenesis and segmentation clock dynamics during vertebrate anteroposterior axis elongation. We find an extension force generated through the posterior notochord that is strong enough to buckle the structure. Our data suggest that this force generates a unidirectional notochord extension towards the tailbud because PSM tissue around the posterior notochord does not let it slide anteriorly. These results complement existing biomechanical models of axis elongation, revealing a critical coupling between the posterior notochord, the tailbud, and the PSM, and show that somite patterning is robust against structural perturbations.

2022

Actuated 3D microgels for single cell mechanobiology

Actuated 3D microgels for single cell mechanobiology (vol 22, pg 1962, 2022)

Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation

Actuated 3D microgels for single cell mechanobiology

Actuated 3D microgels for single cell mechanobiology (vol 22, pg 1962, 2022)

Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation