Publications de Michelle Susanne Frei

Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy

The specific and covalent labeling of the protein HaloTag with fluorescent probes in living cells makes it a powerful tool for bioimaging. However, the irreversible attachment of the probe to HaloTag precludes imaging applications that require transient bi ...

AMER CHEMICAL SOC2023

Engineered HaloTag variants for fluorescence lifetime multiplexing

Kai Johnsson, Michelle Susanne Frei

HaloTag variants offer distinct brightness and fluorescence lifetimes compared with HaloTag7 when labeled with rhodamines. These variants were used for multiplexed imaging with a single fluorophore and to create lifetime-based cell cycle indicators. ...

NATURE PORTFOLIO2022

Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

Michelle Susanne Frei

Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...

EPFL2020

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

Kai Johnsson, Aleksandar Salim, Lu Wang, Michelle Susanne Frei

Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spa ...

AMER CHEMICAL SOC2019

Photoactivation of silicon rhodamines via a light-induced protonation

Kai Johnsson, Luc Reymond, Michelle Susanne Frei

Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to ...

Nature Research2019

The detection of cavitation in hydraulic machines by use of ultrasonic signal analysis

Mohamed Farhat, Pascal Damian Odermatt, Michelle Susanne Frei

This presentation describes an experimental approach for the detection of cavitation in hydraulic machines by use of ultrasonic signal analysis. Instead of using the high frequency pulses (typically 1MHz) only for transit time measurement different other s ...

International Association For Hydraulic Research2014