Publications de Khalid Ashraf Mohie Ibrahim

Self-driving microscopy detects the onset of protein aggregation and enables intelligent Brillouin imaging

Hilal Lashuel, Aleksandra Radenovic, Lely Feletti, Khalid Ashraf Mohie Ibrahim

The process of protein aggregation, central to neurodegenerative diseases like Huntington’s, is challenging to study due to its unpredictable nature and relatively rapid kinetics. Understanding its biomechanics is crucial for unraveling its role in disease ...

Springer Science and Business Media LLC2025

Intelligent and Self-Driving Microscopy of Protein Aggregation in Neurodegenerative Diseases

Khalid Ashraf Mohie Ibrahim

Neurodegenerative diseases, such as Alzheimer's, Parkinson's, and Huntington's, afflict tens of millions of patients worldwide. They are characterized by protein aggregation and progressive neuronal loss, leading to cognitive and motor impairments, and ult ...

EPFL2025

Label-Free Techniques for Probing Biomolecular Condensates

Aleksandra Radenovic, Wayne Yang Wen Wei, Khalid Ashraf Mohie Ibrahim, Helena Miljkovic, Akhil Sai Naidu

Biomolecular condensates play important roles in a wide array of fundamental biological processes, such as cellular compartmentalization, cellular regulation, and other biochemical reactions. Since their discovery and first observations, an extensive and e ...

Amer Chemical Soc2024

AI-driven detection and analysis of label-free protein aggregates

Khalid Ashraf Mohie Ibrahim

In this Tools of the Trade article, Khalid Ibrahim (Radenovic and Lashuel labs) describes a tool for the artificial intelligence (AI)-driven detection of cellular aggregates that bypasses the need for fluorescent labelling. ...

2024

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Khalid Ashraf Mohie Ibrahim, Davide Gambarotto, Paul Guichard, Virginie Hamel, Niccolo Banterle, Dora Mahecic, Denis Fortun

Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...

2020

Characterization of flat-fielding systems for quantitative microscopy

Suliana Manley, Dora Mahecic, Khalid Ashraf Mohie Ibrahim

Optical flat-fielding systems, such as field-mapping or integration-based beam shapers, are used to transform nonuniform illumination into uniform illumination. Thus, flat-fielding paves the way for imaging that is independent of position within a field of ...

2020