Publications de Maksim Eremchev

Membrane Potential Fluctuations and Water Asymmetry on Plasma Cell and Model Lipid Membranes: Origins, Implications and Properties

Sylvie Roke, Arianna Marchioro, Orly Bagunu Tarun, Seonwoo Lee, Mischa Samuel Flór, Iwona Swiderska, Maksim Eremchev, David Roesel, Bryan Zhi Li

Membrane potential fluctuations have previously been detected using second harmonic (SH) water imaging on neuronal cells and model lipid bilayer membranes. We report that such fluctuations are also visible when membrane potential-sensitive fluorophores are ...

ROYAL SOC CHEMISTRY2025

High throughput wide field second harmonic imaging of giant unilamellar vesicles

Sylvie Roke, Maksim Eremchev, David Roesel, Pierre-Marc Jean Marie Dansette

Cell-sized giant unilamellar vesicles (GUVs) are an ideal tool for understanding lipid membrane structure and properties. Label-free spatiotemporal images of their membrane potential and structure would greatly aid the quantitative understanding of membran ...

AIP Publishing2023

Dynamic second-harmonic imaging of divalent cation translocation through giant vesicle membranes

Sylvie Roke, Maksim Eremchev, David Roesel

SPRINGER2023

Passive transport of Ca2+ ions through lipid bilayers imaged by widefield second harmonic microscopy

Sylvie Roke, Maksim Eremchev, David Roesel

In biology, release of Ca2+ ions in the cytosol is essential to trigger or control many cell functions. Calcium signaling acutely depends on lipid membrane permeability to Ca2+. For proper understanding of membrane permeability to Ca2+, both membrane hydra ...

2023

Ion-Induced Transient Potential Fluctuations Facilitate Pore Formation and Cation Transport through Lipid Membranes

Sylvie Roke, Maksim Eremchev, David Roesel

Unassisted ion transport through lipid membranes plays a crucial role in many cell functions without which life would not be possible, yet the precise mechanism behind the process remains unknown due to its molecular complexity. Here, we demonstrate a dire ...

2022

Continuous compensation of the phase mismatch by using temperature gradients for second harmonic generation

Maksim Eremchev

The second harmonic of an infrared laser generated by frequency doubling in a nonlinear crystal can be adversely affected by group delay dispersion applied to the fundamental radiation. Yet large amounts of group delay dispersion can be advantageous when u ...

ELSEVIER2021

Investigation of materials for supercontinuum generation for subsequent nonlinear parametrical and Raman amplification at 1 MHz repetition rate

Maksim Eremchev, Pierre-Marc Jean Marie Dansette

In the present work we performed research of supercontinuum generation in several commonly used and new supercontinuum generation crystals for subsequent nonlinear amplification, using 1-3 mu J energy pulses of 300 fs duration at 1 MHz repetition rate. Obt ...

ELSEVIER SCI LTD2021

Passive and active ion permeation through lipid bilayers investigated by second harmonic water imaging

Maksim Eremchev

Lipid membranes are complex and dynamic systems which are known to mediate signaling processes between cells and their environment. To do this multiple ion channels and pumps are involved in controlling the in- and out-flux of various ions (K+, Na+, Mg2+, ...

EPFL2021

Spatiotemporal Imaging of Water in Operating Voltage-Gated Ion Channels Reveals the Slow Motion of Interfacial Ions

Sylvie Roke, Aleksandra Radenovic, Orly Bagunu Tarun, Maksim Eremchev

Ion channels are responsible for numerous physiological functions ranging from transport to chemical and electrical signaling. Although static ion channel structure has been studied following a structural biology approach, spatiotemporal investigation of t ...

American Chemical Society (ACS)2019

Interaction of Oil and Lipids in Freestanding Lipid Bilayer Membranes Studied with Label-Free High-Throughput Wide-Field Second-Harmonic Microscopy

Sylvie Roke, Orly Bagunu Tarun, Maksim Eremchev

The interaction of oils and lipids is relevant for membrane biochemistry since the cell uses bilayer membranes, lipid droplets, and oily substances in its metabolic cycle. In addition, a variety of model lipid membrane systems, such as freestanding horizon ...

2018