Publications de Nicolas Antoine Vincent Broguiere

Accessible homeostatic gastric organoids reveal secondary cell type-specific host-pathogen interactions in Helicobacter pylori infections

Matthias Lütolf, Nicolas Antoine Vincent Broguiere, Moritz Hofer

Despite the high prevalence of gastric diseases like gastric cancer and peptic ulcer disease attributed to Helicobacter pylori infections, there is still only a limited understanding of the underlying mechanisms. Existing in vitro models are either two-dim ...

NATURE PORTFOLIO2025

Probing the killing potency of tumor-infiltrating lymphocytes on microarrayed colorectal cancer tumoroids

Matthias Lütolf, Krisztian Homicsko, Nathalie Brandenberg, Nicolas Antoine Vincent Broguiere, Sylke Höhnel, François Réal Rivest, Damien Saugy, Devanjali Dutta

Immunotherapy has emerged as a new standard of care for certain cancer patients with specific cellular and molecular makeups. However, there is still an unmet need for ex vivo models able to readily assess the effectiveness of immunotherapeutic treatments ...

2024

Bioengineered human colon organoids with in vivo-like cellular complexity and function

Nicolas Antoine Vincent Broguiere

Organoids and organs-on-a-chip have emerged as powerful tools for modeling human gut physiology and disease in vitro. Although physiologically relevant, these systems often lack the environmental milieu, spatial organization, cell type diversity, and matur ...

2024

Patient-derived mini-colons enable long-term modeling of tumor–microenvironment complexity

Matthias Lütolf, Nicolas Antoine Vincent Broguiere

Existing organoid models fall short of fully capturing the complexity of cancer because they lack sufficient multicellular diversity, tissue-level organization, biological durability and experimental flexibility. Thus, many multifactorial cancer processes, ...

2024

Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo

Freddy Radtke, Matthias Lütolf, Nicolas Antoine Vincent Broguiere, Tania Hübscher, Luis Francisco Lorenzo Martin, Amber Dawn Bowler

Three-dimensional organoid culture technologies have revolutionized cancer research by allowing for more realistic and scalable reproductions of both tumour and microenvironmental structures 1-3 . This has enabled better modelling of low-complexity cancer ...

NATURE PORTFOLIO2024

Nanoplasmonic Single-Tumoroid Microarray for Real-Time Secretion Analysis

Hatice Altug, Nicolas Antoine Vincent Broguiere, Yen-Cheng Liu, Saeid Ansaryan, Luis Francisco Lorenzo Martin

Organoid tumor models have emerged as a powerful tool in the fields of biology and medicine as such 3D structures grown from tumor cells recapitulate better tumor characteristics, making these tumoroids unique for personalized cancer research. Assessment o ...

2024

Dynamic Hydrogel Design For Spatiotemporal Control Of Morphogenesis

Matthias Lütolf, Nicolas Antoine Vincent Broguiere, Antonius Chrisnandy

MARY ANN LIEBERT, INC2023

A New Approach to Generate Gastruloids to Develop Anterior Neural Tissues

Matthias Lütolf, Nicolas Antoine Vincent Broguiere

Embryonic development is a complex process integrating cell fate decisions and morphogenesis in a spatiotemporally controlled manner. Previous studies with model organisms laid the foundation of our knowledge on post-implantation development; however, stud ...

BIO-PROTOCOL2023

Next-generation cancer organoids

Matthias Lütolf, Nicolas Antoine Vincent Broguiere

Organotypic models of patient-specific tumours are revolutionizing our understanding of cancer heterogeneity and its implications for personalized medicine. These advancements are, in part, attributed to the ability of organoid models to stably preserve ge ...

NATURE PORTFOLIO2022

Dissecting mechanisms of chamber-specific cardiac differentiation and its perturbation following retinoic acid exposure

Matthias Lütolf, Nicolas Antoine Vincent Broguiere, Giuliana Rossi

The specification of distinct cardiac lineages occurs before chamber formation and acquisition of bona fide atrial or ventricular identity. However, the mechanisms underlying these early specification events remain poorly understood. Here, we performed sin ...

COMPANY BIOLOGISTS LTD2022