Personne
Andrea Bertarello
Cette personne n’est plus à l’EPFL
Publications associées (10)
Hierarchy of the Components in Spray-Dried, Protein-Excipient Particles Using DNP-Enhanced NMR Spectroscopy
David Lyndon Emsley, Pierrick Berruyer, Andrea Bertarello, Baptiste Busi
Protein-based drugs are becoming increasingly important, but there are challenges associated with their formulation (for example, formulating stable inhalable aerosols while maintaining the proper long-term stability of the protein). Determining the morpho ...
Washington2023H-1 Detected Relayed Dynamic Nuclear Polarization
David Lyndon Emsley, Moreno Lelli, Pierrick Berruyer, Andrea Bertarello
Recently, it has been shown that methods based on the dynamics of 1 H nuclear hyperpolarization in magic angle spinning (MAS) NMR experiments can be used to determine mesoscale structures in complex materials. However, these methods suffer from low sensiti ...
AMER CHEMICAL SOC2022In-Cell Quantification of Drugs by Magic-Angle Spinning Dynamic Nuclear Polarization NMR
David Lyndon Emsley, Pierrick Berruyer, Andrea Bertarello
The determination of intracellular drug concentrations can provide a better understanding of the drug function and efficacy. Ideally, this should be performed nondestructively, with no modification of either the drug or the target, and with the capability ...
AMER CHEMICAL SOC2022Similarities and Differences among Protein Dynamics Studied by Variable Temperature Nuclear Magnetic Resonance Relaxation
David Lyndon Emsley, Jayasubba Reddy Yarava, Andrea Bertarello, Baptiste Busi, François Freymond
Understanding and describing the dynamics of proteins is one of the major challenges in biology. Here, we use multifield variable-temperature NMR longitudinal relaxation (R-1) measurements to determine the hierarchical activation energies of motions of fou ...
AMER CHEMICAL SOC2021Quantification of magic angle spinning dynamic nuclear polarization NMR spectra
David Lyndon Emsley, Pierrick Berruyer, Andrea Bertarello
Dynamic nuclear polarization (DNP) allows to dramatically enhance the sensitivity of magic angle spinning nuclear magnetic resonance (MAS NMR). DNP experiments usually rely on the detection of low-γ nuclei hyperpolarized from 1H with the use of cross polar ...
2021Dynamic Nuclear Polarization Enhancement of 200 at 21.15 T Enabled by 65 kHz Magic Angle Spinning
David Lyndon Emsley, Yu Rao, Moreno Lelli, Pierrick Berruyer, Gabriele Stevanato, Snaedis Björgvinsdóttir, Andrea Bertarello
Solid-state nuclear magnetic resonance under magic angle spinning (MAS) enhanced with dynamic nuclear polarization (DNP) is a powerful approach to characterize many important classes of materials, allowing access to previously inaccessible structural and d ...
2020Picometer Resolution Structure of the Coordination Sphere in the Metal-Binding Site in a Metalloprotein by NMR
David Lyndon Emsley, Andrea Bertarello
Most of our understanding of chemistry derives from atomic-level structures obtained with single-crystal X-ray diffraction. Metal centers in X-ray structures of small organometallic or coordination complexes are often extremely well-defined, with errors in ...
2020Structure of fully protonated proteins by proton-detected magic-angle spinning NMR
David Lyndon Emsley, Andrea Bertarello
Protein structure determination by proton-detected magic-angle spinning (MAS) NMR has focused on highly deuterated samples, in which only a small number of protons are introduced and observation of signals from side chains is extremely limited. Here, we sh ...
National Academy of Sciences2016Protein residue linking in a single spectrum for magic-angle spinning NMR assignment
David Lyndon Emsley, Andrea Bertarello
Here we introduce a new pulse sequence for resonance assignment that halves the number of data sets required for sequential linking by directly correlating sequential amide resonances in a single diagonal-free spectrum. The method is demonstrated with both ...
Springer Verlag2015Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning
David Lyndon Emsley, Andrea Bertarello
Using a set of six H-1-detected triple-resonance NMR experiments, we establish a method for sequence-specific backbone resonance assignment of magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectra of 5-30 kDa proteins. The approach relies on ...
AMER CHEMICAL SOC2014