Beyond the lateral resolution limit by phase imaging

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Live-cell imaging powered by computation

Suliana Manley, Florian Jug

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

Nature Portfolio2024

Fluorescence microscopy: A statistics-optics perspective

Aleksandra Radenovic

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scal ...

Amer Physical Soc2024

Ghost imaging using two SPAD array detectors: a parameter study towards the realization of a 3D quantum microscope

Edoardo Charbon, Claudio Bruschini, Paul Mos, Yang Lin

Quantum ghost imaging can be an important tool in making optical measurements. One of the most useful aspects of ghost imaging is the unique ability to correlate two sets of independently collected information. We aim to use the principles of ghost imaging ...

Spie-Int Soc Optical Engineering2024

Advances in High-Speed, Multiparametric Atomic Force Microscopy

Santiago Harald Andany

After decades of technological advancements, high-speed atomic force microscopy (HS-AFM) has emerged as a powerful technique for visualizing dynamic processes. At the nanoscale, the AFM provides valuable insights into the sample by sensing minute interacti ...

EPFL2024

Compact and effective photon-resolved image scanning microscope

Giorgio Tortarolo

Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spati ...

Spie-Soc Photo-Optical Instrumentation Engineers2024

3D printed large amplitude torsional microactuators powered by ultrasound

Mahmut Selman Sakar, Mehdi Ali Gadiri, Junsun Hwang, Amit Yedidia Dolev

Here, we introduce a design, fabrication, and control methodology for large amplitude torsional microactuators powered by ultrasound. The microactuators are 3D printed from two polymers with drastically different elastic moduli as a monolithic compliant me ...

2024

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü, Yichen Feng

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

SPIE Society of Photo-optical Instrumentation Engineers2024

Tangentially viewing phase contrast imaging for turbulence measurements in toroidal fusion devices

Stefano Coda

Phase contrast imaging (PCI) is an established and powerful technique for measuring density fluctuations in plasmas and has been successfully applied to several fusion devices. Rooted in a concept first developed for microscopy, PCI belongs to the category ...

2024

3D diffractive optics for linear interconnects and nonlinear processing

Niyazi Ulas Dinç

The optical domain presents potential avenues for enhancing both computing and communication due to its inherent properties of bandwidth, parallelism, and energy efficiency. This research focuses on harnessing 3-Dimensional (3D) diffractive optics for nove ...

EPFL2024

Surface passivation and functionalisation for mass photometry

Suliana Manley, Jenny Sülzle, Laila Abdelaziz Abdelmoniem Elfeky

Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecules. Consequently, single-particle imaging and tracking with the iSCAT-based method known as mass photometry (MP) is a growing area of study. However, establishing ...

Wiley2024

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Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü, Yichen Feng

SPIE Society of Photo-optical Instrumentation Engineers2024