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A review, with 17 refs. Methods for the regioselective introduction of alkoxymethyl-groups into the 2'-O-position of 5'-O-dimethoxytritylated, nucleobase-protected ribonucleosides and for the N-alkyloxycarbonylation of adenine and guanine nucleosides were ...
A microfluidic device for random fragmentation of DNA or RNA based on the application of a hydrodynamic shear force to these biomolecules is presented. Different from conventional fragmentation instruments, the microchip-based implementation allows molecul ...
The reaction of d,l-5'-activated nucleotide of adenosine in the presence and absence of montmorillonite gave 60:40 and 96:4 ratios, resp. of the d,d- and l,l:d,l- and l,d-dimers. [on SciFinder (R)] ...
We present the synthesis of a series of deuterated cryptophanes 2-6 by a slightly modified procedure used for cryptophane-A. We show that for [Xe@cryptophane] complexes the use of variable-temperature one-dimensional Xe-129 magnetization transfer (1D-EXSY) ...
Two sets of RNA phosphoramidites, carrying the (fluoride-labile) 2'-O-[(triisopropylsilyl)oxy]methyl (tom) group and the (photolabile) [(R)-1-(2-nitrophenyl)ethoxy]methyl ((R)-npeom) group, were prepd. The two protecting groups were completely orthogonal t ...
A review and discussion, with 42 refs. Base pairing in p-RNA (b-D-ribopyranosyl-(4'->2')-oligonucleotides) is not only stronger than in DNA and RNA, but also more selective in the sense that it is strictly confined to the Watson-Crick mode. Homopurine sequ ...
Telomerase is a ribonucleoprotein enzyme that adds repetitive sequences to the ends of linear chromosomes, thereby counteracting nucleotide loss due to incomplete replication. A short region of the telomerase RNA subunit serves as template for nucleotide a ...
A method for the introduction of the 2'-O-[(triisopropylsilyl)oxy]methyl (=tom) group into N-acetylated, 5'-O-dimethoxytritylated ribonucleosides is presented. The corresponding 2'-O-tom-protected phosphoramidite building blocks were obtained in pure form ...
A synthetic method for the N-alkyloxycarbonylation of adenine and guanine nucleosides was developed and used for the prepn. of RNA-phosphoramidites carrying photolabile sugar and nucleobase protecting groups. From these building blocks, a heptameric oligor ...
Telomerase is a ribonucleoprotein enzyme that uses its internal RNA moiety as a template for synthesis of telomeric repeats at chromosome ends. Here we report the purification of telomerase from Euplotes aediculatus by affinity chromatography with antisens ...
