Time-resolved spectrofluorometer for clinical tissue characterization during endoscopy
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Fibers doped with bismuth show broad luminescence bands around 750, 1100, and 1400 nm with spectral bandwidths of 100 to 200 nm at room temperature. The luminescence bands can be observed by pumping broad absorption bands in the visible or near infrared sp ...
The Biotin-Streptavidin complex is a widely studied system in biology and biophysics, because of its extremely strong non-covalent binding affinity. The latter is often exploited to link molecules to substrates or to one another. However, the details of th ...
We incorporate newly developed solid-state detector technology into time-resolved laser Raman spectroscopy, demonstrating the ability to distinguish spectra from Raman and fluorescence processes. As a proof of concept, we show fluorescence rejection on hig ...
A new optical-fiber-based spectrofluorometer for in vivo or in vitro detection of delayed fluorescence is presented and characterized. This compact setup is designed so that it can be readily adapted for future clinical use. Optical excitation is done with ...
The high sensitivity of fluorescence imaging enables the detection of molecular processes in living organisms. However, diffuse light propagation in tissue prevents accurate recovery of tomographic information on fluorophore distribution for structures emb ...
Institute of Electrical and Electronics Engineers2011
Despite the experience gained over several decades in various types of light-based medical treatments, the optimization of the corresponding therapeutic protocols and accurate forecasting of their outcome have not yet been achieved in many cases. The diffi ...
A coumarin derivative was employed for the detection of biogenic amines in buffered aqueous solution by UV-Vis or fluorescence spectroscopy. Incorporated in a polymeric matrix, the dye can also be used for the optical detection of gaseous amines. ...
The optical properties of small size selected metal clusters have been studied by fluorescence spectroscopy. Cun (n = 1-5, 8), Agn (n = 1-5, 8, 9, 20), Aun (n = 1-5, 8) and Nin (n = 1-3) clusters embedded in condensed neon are photoionized by VUV photons i ...
We demonstrate temporally shaped pulses in the deep ultraviolet spectral range (270 nm) with energies up to 37 mu J using an efficient prism stretcher and an acousto-optic programmable dispersive filter (AOPDF) applied directly in the UV. The scheme allows ...
The excited-state dynamics of five derivatives of the GFP-chromophore, which differ by the position and nature of their substituents, has been investigated in solvents of various viscosity and polarity and in rigid media using femtosecond-resolved spectros ...