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Regulation of transferrin receptor mRNA expression. Distinct regulatory features in erythroid cells

Publications associées (35)

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Role of Ets-1 in transcriptional regulation of transferrin receptor and erythroid differentiation

Lukas Kühn

High expression of transferrin receptor (TfR) on the membrane of erythroid cells accounts for the high level of iron required to sustain heme synthesis. Several studies indicate that during erythroid differentiation TfR expression is highly dependent on tr ...

2002

Determination of drug binding sites to proteins by electrospray ionisation mass spectrometry: the interaction of cisplatin with transferrin

Paul Joseph Dyson, Claire Samantha Allardyce

Cisplatin, CiS-[PtCl2(NH3)(2)], is known to bind to human serum transferrin, but the binding site remains a matter of some debate. Electrospray ionisation mass spectrometry has been used to characterise the interaction of cisplatin with transferrin. The st ...

2002

We have investigated the expression of transferrin receptor (TfR) iron regulatory protein-1 (IRP-1) and iron regulatory protein-2 (IRP-2) in liquid suspension culture of purified hematopoietic progenitor cells (HPCs) induced by a growth factor stimulus to ...

2000

Identification of RNA-binding surfaces in iron regulatory protein-1

Lukas Kühn

Post-transcriptional regulation of mRNA translation and stability in iron metabolism involves the interaction between the trans-acting cytoplasmic iron regulatory proteins (IRP-1 and IRP-2) and cis-acting iron-responsive elements (IREs) in mRNA 5'- or 3'-u ...

1999

Iron regulatory proteins (IRPs) control the synthesis of several proteins in iron metabolism by binding to iron-responsive elements (IREs), a hairpin structure in the untranslated region (UTR) of corresponding mRNAs. Binding of IRPs to IREs in the 5' UTR i ...

1999

Translational regulation of mRNAs with distinct IRE sequences by iron regulatory proteins 1 and 2

Lukas Kühn

Iron regulatory proteins 1 and 2 (IRP-1, IRP-2) interact with iron-responsive elements (IREs) present in the 5'- or 3'-untranslated regions (UTR) of several mRNAs coding for proteins in iron metabolism. Whereas binding of IRP-1 and -2 to an IRE in the 5'-U ...

1998

Transferrin receptor expression is controlled differently by transferrin-bound and non-transferrin iron in human cells

Lukas Kühn

We studied the effects of iron supplied as transferrin-bound iron and iron supplied as non-transferrin iron on transferrin receptor expression by human cell lines. Defined conditions of iron supply were represented by (i) 5 microg/ml of iron-saturated tran ...

1998

The regulation of transferrin-receptor synthesis was studied in J2E erythroid cells induced to differentiate with erythropoietin. Nuclear run-on assays demonstrated that transcription of the transferrin-receptor gene rose markedly after erythropoietin trea ...

1997

The inability of cells to grow in low iron correlates with increasing activity of their iron regulatory protein (IRP)

Lukas Kühn

We studied the factors that determine the differing growth requirements of low-iron-tolerant (LIT) versus high-iron-dependent (HID) cells for extracellular nontransferrin iron. The growth of LIT cells HeLa and THP-1, when transferred from transferrin (5 mi ...

1997

Iron regulatory proteins 1 and 2 bind distinct sets of RNA target sequences

Lukas Kühn

Iron regulatory proteins (IRPs) 1 and 2 bind with equally high affinity to iron-responsive element (IRE) RNA stem-loops located in mRNA untranslated regions and, thereby, post-transcriptionally regulate several genes of iron metabolism. In this study we de ...

1996