Publication
Genotypic features of lentivirus transgenic mice
Publications associées (46)
Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency
Didier Trono, Priscilla Turelli, Evaristo Jose Planet Letschert, Bastien Mangeat, Marc Samuel Friedli, Adamantia Kapopoulou, Gabriela Ecco, Helen Mary Rowe, Benjamin Rauwel, Carmen Maria Unzu Ezquerro
Endogenous retroelements (EREs) account for about half of the mouse or human genome, and their potential as insertional mutagens and transcriptional perturbators is suppressed by early embryonic epigenetic silencing. Here, we asked how ERE control is maint ...
Cold Spring Harbor Lab Press, Publications Dept2014Hydrogel microfluidics for the patterning of pluripotent stem cells
Matthias Lütolf, Steffen Cosson
Biomolecular signaling is of utmost importance in governing many biological processes such as the patterning of the developing embryo where biomolecules regulate key cell-fate decisions. In vivo, these factors are presented in a spatiotemporally tightly co ...
Nature Publishing Group2014TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells
Didier Trono, Adamantia Kapopoulou, Andrea Corsinotti, Helen Mary Rowe, Johan Jakobsson
TRIM28 is critical for the silencing of endogenous retroviruses (ERVs) in embryonic stem (ES) cells. Here, we reveal that an essential impact of this process is the protection of cellular gene expression in early embryos from perturbation by cis-acting act ...
2013Global and stage specific patterns of krüppel-associated-box zinc finger protein gene expression in murine early embryonic cells
Didier Trono, Bart Deplancke, Adamantia Kapopoulou, Michaël Imbeault, Andrea Corsinotti, Helen Mary Rowe, Yoann Mouscaz
Highly coordinated transcription networks orchestrate the self-renewal of pluripotent stem cell and the earliest steps of mammalian development. KRAB-containing zinc finger proteins represent the largest group of transcription factors encoded by the genome ...
Public Library of Science2013A Genetic Approach to the Recruitment of PRC2 at the HoxD Locus
Denis Duboule, Fabrice Daniel Darbellay, Patrick Schorderet, Nicolas Lonfat
Polycomb group (PcG) proteins are essential for the repression of key factors during early development. In Drosophila, the polycomb repressive complexes (PRC) associate with defined polycomb response DNA elements (PREs). In mammals, however, the mechanisms ...
Public Library of Science2013De novo DNA methylation of endogenous retroviruses is shaped by KRAB-ZFPs/KAP1 and ESET
Didier Trono, Sonia Verp, Marc Samuel Friedli, Sandra Eloise Kjeldsen, Helen Mary Rowe
Endogenous retroviruses (ERVs) undergo de novo DNA methylation during the first few days of mammalian embryogenesis, although the factors that control the targeting of this process are largely unknown. We asked whether KAP1 (KRAB-associated protein 1) is i ...
Company Of Biologists Ltd2013Polycomb Recruitment and Targeting in Mammals
Polycomb- (PcG) and trithorax (trxG) group proteins are essential components in the regulation of multiple developmental regulators. In particular, during early stages, they act as chromatin modifiers in such a way as to set and maintain the transcriptiona ...
EPFL2013Cellular Heterogeneity During Embryonic Stem Cell Differentiation to Epiblast Stem Cells Is Revealed by the ShcD/RaLP Adaptor Protein
Daniel Constam, Anja Dietze, Antonio Simeone
The Shc family of adaptor proteins are crucial mediators of a plethora of receptors such as the tyrosine kinase receptors, cytokine receptors, and integrins that drive signaling pathways governing proliferation, differentiation, and migration. Here, we rep ...
Wiley-Blackwell2012Embryonic stem cell potency fluctuates with endogenous retrovirus activity
Embryonic stem (ES) cells are derived from blastocyst-stage embryos and are thought to be functionally equivalent to the inner cell mass, which lacks the ability to produce all extraembryonic tissues. Here we identify a rare transient cell population withi ...
2012Development of a microfluidic on-chip reverse transcription module for the analysis of hematopoietic single stem cells
Hematopoietic stem cells (HSCs) have a unique ability to self-renew and produce differentiated progeny throughout the entire life of the human body. However, present clinical use of HSCs in regenerative medicine is largely hindered by our limited understan ...
2011