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Dual-wavelength digital holographic microscopy with sub-nanometer axial accuracy

Publications associées (32)

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Correlating microscopy techniques and ToF-SIMS analysis of fully grown mammalian oocytes

Sami Rtimi

The 2D-molecular thin film analysis protocol for fully grown mice oocytes is described using an innovative approach. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and optical mi ...

Royal Soc Chemistry2016

Nanoscopy in nonlinear scanning fluorescence imaging systems

Grégoire Pierre Jean Laporte

In the last 30 years, superresolution in optical microscopy has been a major field of research. During this time, different techniques have been created to break the diffraction limit in order to make observations at a nanometric scale. Given that optical ...

EPFL2016

Broadband Digital Holographic Camera for Microscopy

Zahra Monemhaghdoust

In this thesis, we explore a combination of analog and digital holographic techniques to demonstrate high speed quantitative phase imaging (QPI) using low coherence (broad bandwidth) light sources. Off-axis digital holographic microscopy is inherently a co ...

EPFL2015

DEA-based deformable cell culture system

Herbert Shea, Cristina Martin Olmos, Samuel Rosset, Alexandre Poulin, Martha Liley

We present a deformable cell culture system based on dielectric elastomer actuator (DEA). Understanding how the mechanical environment can affect cells functions could lead to significant advances in diseases diagnosis and drug development. Most available ...

2014

Digital holographic confocal microscope

Demetri Psaltis, Alexandre Goy

We demonstrate experimentally a scanning confocal microscopy technique based on digital holographic recording of the scanned spot. The data collected in this way contains all the information to produce three-dimensional images. Several methods to treat the ...

Spie-Int Soc Optical Engineering2013

Single-cell lysis for visual analysis by electron microscopy

Henning Paul-Julius Stahlberg

The stochastic nature of biological systems makes the study of individual cells a necessity in systems biology. Yet, handling and disruption of single cells and the analysis of the relatively low concentrations of their protein components still challenges ...

Elsevier BV2013

Morphological investigation of polylactide/microfibrillated cellulose composites

Pierre-Etienne Bourban, John Christopher Plummer, Carole Boissard

Optical microscopy and transmission electron microscopy have been used to investigate the morphology of polylactide (PLA)/microfibrillated cellulose (MFC) composites prepared by: compression molding of wet-comingled MFC and PLA latex or powder, twin-screw ...

Springer Berlin Heidelberg2013

Digital confocal microscope

Demetri Psaltis, Alexandre Goy

We demonstrate experimentally a scanning confocal microscopy technique based on digital holography. The method relies on digital holographic recording of the scanned spot. The data collected in this way contains all the necessary information to digitally p ...

Optical Society of America2012

Resolution and Complex Deconvolution in Holographic Microscopy

Yann Jérôme Michel Pascal Cotte

Interest in high resolution imaging techniques has recently multiplied due to their importance in bio-medical research. Quantitative phase measurements by holographic microscopy is an extraordinary tool to gain new understanding of transparent biological s ...

EPFL2012

Beyond the lateral resolution limit by phase imaging

Christian Depeursinge, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We present a theory stating how to overcome the classical Rayleigh-resolution limit. It is based upon a new resolution criterion in phase of coherent imaging process and its spatial resolution is thought to be only SNR limited. Recently, the experimental o ...

SPIE2011